【作者】 杨志勇；

【导师】 徐涛； 瞿安连；

【作者基本信息】 华中科技大学 ， 生物医学工程， 2004， 硕士

【摘要】 钙离子作为细胞内重要的第二信使,调控着许多细胞生理活动。以荧光显微镜为基础的钙离子浓度检测系统是生物物理学研究中重要的实验设备,其中的单色光源系统,国内有关的技术研究和仪器制造尚属空白。本文通过对单色光源系统关键技术的理论模型分析,得到设计方案并最终研制出样机用于实验。设计的单色光源系统采用短弧氙灯作为发光源,利用椭球面反射镜聚焦后得到模拟的点光源,经过准直,闪耀光栅分光,汇聚后,耦合到光纤中形成单色光输出。单色光源的输出功率和光谱纯度是仪器的两个技术关键,本文通过建立数学模型的方法,对系统进行模拟分析,给出了优化的分析结果。在理论分析的基础上,进行了相关光学设计和机械设计,确定了最终的方案。研制的单色光源和荧光检测系统经过校准后,在荧光测钙实验中得到应用,效果良好。本文的主要研究内容分为以下三个部分:第一部分介绍了单色光源系统的基本结构,以及相关光学元件的选择依据和设计方法。第二部分利用数学模型,分析了单色光源系统的能量输出和光谱纯度,给出了仿真模拟结果,以及闪耀光栅的工作原理,为系统方案设计提供了理论依据。第三部分介绍了整个系统的调试原理和方法,并给出了在本系统下所做的高钾去极化刺激下PC12细胞(大鼠嗜铬细胞瘤细胞)内钙离子浓度测量实验的方法和结果,验证了单色光源系统的实用性。更多还原

【Abstract】 As the important intracellular secondary messenger, Ca2+ regulates many physiological activities of cells. The [Ca2+]i detecting system, which is based on the fluorescence microscopy, is an important experiment equipment in biophysics research. But the technical research and manufacture of monochromator are lagged in our country. This article gives a design to monochromator by simulating and analyzing the mathematical model, and the demo device has been used in the [Ca2+]i detecting experiment.The monochromator uses short-arc Xenon lamp as the light source. All the light is converged to a point light source by the elliptical mirror. During the course of collimation, grating diffraction, focus and coupling, the monochromatic light output from the optical fiber. The light power and spectral purity are the tow technical essentials of the monochromator. Using mathematical model to simulate the monochromator system, the optical and mechanical design could be made by the emulation result. After calibration, the monochromator is used in [Ca2+]i detecting experiment and gets eligible result.The research content in this paper could be divided into three parts.The first part introduces the principle and structure of monochromator, the choosing and design methods of the optical components.The second part analyses the output power and spectral purity by mathematical model. The simulation result gives theoretic reasons for optical system design. This part also introduces the work principle of diffraction grating.The third part narrates how to calibrate all parts of the [Ca2+]i detecting system. Using the system, the dynamic curve of [Ca2+]i in PC12 cells after high K+ depolarization is given.更多还原