【作者】 周云燕；

【导师】 徐涛； 瞿安连；

【作者基本信息】 华中科技大学 ， 生物物理学， 2004， 硕士

【摘要】 为了实现胞内钙离子浓度的检测,本文设计了一种新型的胞内钙离子浓度荧光显微检测系统。论文的工作主要有两部分:单色激发光源系统的设计和微弱荧光检测系统的设计。单色激发光源系统中,短弧氙灯发射的光经椭球反射镜聚焦后采用离轴抛物面反射镜准直成平行光,通过光栅的衍射分光后采用三片型会聚物镜把光能量聚焦到光纤端面,光纤的另一端面输出单色光。设计时,采用光路偏折的方法,有效地克服了灯源的噪声,另外,本系统采用易于加工的椭球反射镜代替目前国际上应用比较广泛的超环面镜。微弱荧光检测系统中,用光电倍增管采集荧光探针发出的弱荧光,并转化为电信号,通过增益调节电路和贝塞尔滤波电路对光电倍增管输出的电信号进行放大和滤波。全文的研究内容主要可分为三个部分。第一部分介绍了单色激发光源系统的结构与设计过程,重点讨论分光系统的设计,并得出波长控制关系。第二部分论述了微弱荧光检测系统的设计,采用光电倍增管检测荧光信号。第三部分详细描述了实验过程的安装、校准及验证过程,首先介绍仪器的安装与调试以及波长校准的过程,然后利用该系统进行胞内钙离子浓度的检测,得到满意的实验结果。更多还原

【Abstract】 In order to detect [Ca2+]i, a novel fluorescent microscopy system to detect [Ca2+]i in living cell has been designed. The main work is divided into two sections: the design for monochromator and the design for the system to detect the faint fluorescent signal.In the system of monochromator, the light from the short-arc xenon lamp is first converged by the ellipse mirror, then collimated by paraboloidal mirror, and diffracted by the grating, at last focused into the fiber by triple-lens whose another end export the monochromatic wave. In the design of this system, the optical axis redirection method is employed, efficiently reducing the noise of the lamp, and this system uses the economical ellipse mirror,not the torodial mirror. In this equipment to detect the faint fluorescent signal, the weak fluorescence emitted from fluorescent probes is collected by the PMT, converted to electronic signal that is then amplified and filtered by the amplifier and Bessel filter.The main content of the paper is divided to three sections as follows: The first part concerns the structure and design of the monochromator and the rule of controlling wavelength. The emphases in this part is the design of the system to disperse the light. The second part introduces the design for the system to detect the faint fluorescent signal. In the system, we use PMT to detect the fluorescent signal. The last part particularly describes and the process including system installation and wavelength calibration. Then, using this system, we get the satisfactional experiment results .更多还原