【作者】 王宇飞；

【导师】 赵开弘；

【作者基本信息】 华中科技大学 ， 环境工程， 2004， 硕士

【摘要】 简要介绍了有关生物光电材料及其在光电器件方面的应用研究进展。研究了pH、温度及光照对天然藻红蓝蛋白(亚基((-PEC)的稳定性的影响,并采用sol-gel包埋技术,用四种不同的包埋剂对其进行了包埋固定,结果表明(-PEC在中性条件下,具有较好的pH稳定性、热稳定性、及耐疲劳性,发现琼脂糖是(-PEC较好的固定化包埋剂,经包埋固定的(-PEC稳定性好,包埋工艺简单,剩余光化学活性高,是构建基于(-PEC生物光电器件较好的固定化试剂。 研究了鱼腥藻细菌光敏色素PCC7120 AphA(N490)光诱导下的质子化反应,发现处于Pr态下的AphA(N490)当受到650nm红光照射时,其异构化至Pfr态时,有质子自蛋白质分子向液相转移,蛋白溶液出现酸化效应;处于Pfr态下的AphA(N490)当受到700nm远红光照射时,其异构化至Pr态时,出现质子吸收反应,蛋白溶液回复到以前酸碱度,证明了PCC7120 AphA(N490)当受到光照时可发生质子化反应。本文第四部分采用自组装技术,构建了Au-Cys-PC自组装复合功能膜,并结合光电化学手段研究了藻蓝蛋白光电响应特性。但数十次的实验结果均未发现自组装在金电极表面复合膜上的藻蓝蛋白(PC)当受到复合光照射时出现的光电流响应,终于有一次观察到复合功能膜受光照时出现光电流响应,当撤销光照时,这种光电流消失,具有多循环光敏生物开关特性。如果此观察将来被证实,会为进一步开发基于藻胆蛋白特别是藻蓝蛋白的仿生光电器件打下了基础。更多还原

【Abstract】 Progress in the photoelectronic bio-materials and applications was introduced.Influence of pH value, temperature and illumination on the photochemical activity of (-subunits of phycoerythrocyanin was investigated in this thesis. In comparison with the (-PEC in various carriers such as agar, gelatin, sodium alginate, polyvinyl alcohol-sodium alginate, the agar was selected as better carrier for immobilized (-PEC and was a kind of good chemical for immobilization used in photoelectronic bio-applications assembly based on phycobiliproteins.The protonation/deprotonation reactions triggered by photoactivation of AphA(N490) from cyanobacterium anabaena PCC7120 were investigated. The results presented in this study demonstrated that during the Pr-Pfr transitions of AphA(N490) protons were released from protein to the liquid when induced with 650nm light, while during the Pfr-Pr transitions of AphA(N490) protons were taken up by protein when induced with 700nm light. In addition, the results demonstrated the light-induced proton release and proton uptake reactions in the AphA(N490) from cyanobacterium anabaena PCC7120.Au-Cys-PC composite film was fabricated by self-assembled monolayers (SAMs) on the Au electrode. The photoelectronic characteristics of Au-Cys-PC monolayers were studied. The experiment was carried out for ten times but failed to obtain repeatedly this type of functional monolayer. The photocurrent was observed only once which presented the characteristics of multicycle bio-photoswitch. If this fabrication of the monolayer is repeated in the future, the specific approach reported here supplied a good method to fabricate biomimetic photoelectronic applications based on phycobiliproteins.更多还原