【作者】 杨承；

【导师】 于军；

【作者基本信息】 华中科技大学 ， 微电子学与固体电子学， 2004， 硕士

【摘要】 毛细管电泳芯片具有分析速度快、样品用量少、分离效率高等诸多优点,自问世以来,就得到了人们的普遍关注。在短短十几年的发展时间里,它迅速发展成为一种有效的分析检测手段,在生命科学、分析化学等诸多领域得到了广泛的应用。其中,一维毛细管电泳芯片在基础理论、加工技术、流体控制和检测装置等方面均得到了广泛深入的研究,而在此基础上提出的二维毛细管电泳芯片,将两种不同的分离机制相耦联,因而具有原先任何一种分离方法所无法比拟的分离能力。本论文的研究工作主要包括:一维毛细管电泳芯片的制作与分离实验、二维毛细管电泳芯片的设计和二维芯片制作工艺实验研究等三方面内容。我们使用聚二甲基硅氧烷为材料,加工制作出十字交叉形管道结构的一维毛细管电泳芯片,并在此芯片上进行了初步的DNA片段长度分析实验。实验结果表明,该芯片可以完成对DNA片段的分离分析检测,平均理论塔板数约为16,000,可以将相差10bp的DNA片段分离检测出来,分离效率为0.43。同时,对目前芯片中存在的一些问题进行了比较详细的分析和讨论,并指出了今后的改进方向。在成功实现一维毛细管电泳芯片样品分离实验的基础上,根据二维毛细管电泳芯片的原理,提出了二维毛细管电泳芯片的实验方案,通过对流体运动状态的理论模拟和芯片局部结构的改进,完成了二维毛细管电泳芯片的设计。并根据此设计,对其制作工艺进行了实验研究,实现了二维毛细管电泳芯片的制备。更多还原

【Abstract】 Chip-based capillary electrophoresis (CE) has attracted considerable attention, due to its fast speed, less sample consumption and high resolution. In the past decade, it has become a powerful analytical method and has been applied to many fields, such as life science, analytical chemistry, etc. CE chip has been investigated in-depth at the aspects of basic theory, machining technology, liquid controlling and detection settings. According these, the two-dimensional capillary electrophoresis chip was brought forward. It couples two different separation mechanisms and has a high analysis capability.The work presented in this thesis consists of three parts. One is the fabrication and DNA sizing analysis of CE chip, the second is the design of the two-dimensional capillary electrophoresis chip, and the third is the study of the two-dimensional capillary electrophoresis chip micro fabrication processes.The crossed-channel CE chip which we have constructed, was made of poly(dimethylsiloxane). By using this chip, the primary research of nucleic acid sizing was conducted. The results demonstrated that DNA fragments can be separated and the average theoretical plate number was up to 16,000. The samples with 10 bp differences in length can be separated and the resolution reached 0.43. The problems in present design are also pointed out and discussed in details. Based on the chip-based capillary electrophoresis we have constructed, we designed a two-dimensional capillary electrophoresis chip. With the theoretical calculation of microfluidic control and the optimization of the channel structure, the two-dimensional CE chip was designed.Finally, the fabrication processing of the two-dimensional CE chip was researched, and the manufacture of the chip was presented.更多还原