青霉素发酵过程仿真建模及训练系统

【作者】 项红；

【导师】 吴重光；

【作者基本信息】 北京化工大学 ， 控制理论与控制工程， 2001， 硕士

【摘要】 目前仿真训练系统在化工生产中的应用比较普遍，而在生化过程中却很缺乏。以反应过程复杂、反应周期长的具有典型代表性质的生化反应过程——青霉素发酵为研究对象，建立切实可行的仿真训练系统，对制药业和高等工程教育都有实用意义。 首先，本文对生化反应的发酵方式及其特征进行了详细地分析和阐述，总结了细胞生长动力学方程，并对青霉素发酵过程中的各种模型作了简单地介绍。 综合各种文献资料，在对青霉素发酵过程进行了深入地了解和分析的基础上，总结出发酵过程中主要的外部操作条件影响因素有搅拌转速、空气流量、冷却水流量、培养基流加量、氨水流加量等，根据在发酵过程中的菌体浓度、产物浓度、溶解氧浓度、基质浓度、温度、pH值等变量之间以及这些变量与外部操作之间的关系，建立了能够比较全面地反映青霉素发酵过程各种参数变化的动态数学模型。经过在不同操作条件下的动态运行，不同参数的反应过程趋势与各种文献中所呈现的青霉素发酵过程特征相吻合。 本仿真训练系统使用Visual Basic 6.0进行编程，系统中包括模拟真实操作环境的流程图画面和反映发酵过程中各主要反应变量反应曲线的趋势画面，反应中这两个画面可以任意切换，以便随时了解变量的变化趋势。在实际仿真操作中可以对任一变量进行扰动和调节；反应过程中有报警声响显示功能。 总之，整体仿真系统基本达到了实时性、逼真性和可操作性的要求。更多还原

【Abstract】 At present simulation training system is widely used in chemical plant, but is short of in biochemical process. Penicillin fermentation is one of the most typical biochemical reaction process. Taking penicillin as an object of study which has a complex reaction process and a long reaction cycle, establishes a simulation training system is a very useful work for pharmaceutical industry and higher education of engineering.Above all, a detailed analysis and elaborating of fermentation modes and characteristics is made in this paper, and simply introduce various models of penicillin production.Production of penicillin, and many other secondary metabolites, follows a typical pattern in batch process: a rapid growth phase followed by a second phase of product formation. Roundly considering the speciality of the fermentation process and the changes of various parameters, the mathematical dynamic model is formulated to describe trends in biomass and penicillin formation as well as substrate and oxygen consumption for fed-batch cultivation. The general physic-chemical conditions of the fermentation, such as temperature, pH, flow rate of air, rotational speed of mixer, are taken into account, the model is therefore dependent on these variables.The model is run under different operating conditions. The results of simulation have been shown to exhibit most of the features of penicillin fermentation as reported in the literature, and reflect the general characteristics observed in the penicillin fermentation process.The system is programmed in Visual Basic 6.0. It includes two main menus: the flow chart menu which simulates real operating environment,and diagram of curves menu which reflects the trends of the variables during the fermentation. These two menus can switch to each other at any moment. During the operation any random disturbance and adjustment can be made, and the system is provided with the alarm bell.In conclusion, the whole simulation training system achieves the quality of real time, reality and operability.更多还原