【作者】 俞超；

【导师】 吴平；

【作者基本信息】 浙江大学 ， 植物学， 2004， 硕士

【摘要】 由拟南芥基因组中克隆出衰老相关SEN1基因启动子并融合GUS，转入Columbia野生型拟南芥中。pSEN1：：GUS的转基因拟南芥在正常培养五天后，分别用缺氮、缺磷、缺钾营养胁迫，冷、盐、高渗、黑暗逆境胁迫，加葡萄糖胺处理及ABA、6-BA、NAA激素处理三天。GUS染色结果显示，黑暗处理下SEN1基因在拟南芥叶片中的表达最强烈；缺磷、高渗胁迫、葡萄糖胺和NAA处理下，SEN1在拟南芥根部诱导表达；ABA能诱导SEN1基因在拟南芥侧根基部表达。在自然衰老情况下，SEN1基因在生长30天后的叶、根、花中都能表达。更多还原

【Abstract】 The promoter of SEN1 cloned from Arabidopsis thaliana genome was fused to the GUS reporter and then introduced into Arabidopsis thaliana(Col-l). The expression of SEN1 gene was examined by GUS staining under nutrition stress(nitrogen phosphorus , potassium deficiency), adversity stress (cold, drought, salt, dark), hormone treatment (ABA, 6-BA, NAA )and glucosamine treatment. The result showed that dark could most dramaticly induce SEN1 gene in the Arabidopsis leaves. Phosphorus deficiency drought stress glucosamine and NAA treatment could induce SEN1 gene in Arabidopsis root. ABA could induce SEN1 gene in Arabidopsis lateral root primordia. During the normal growth phase, the gene was induced in leaves, roots, and inflorescence at about 30 days after germination.更多还原