【作者】 刘德利；

【导师】 张恒庆；

【作者基本信息】 辽宁师范大学 ， 植物学， 2004， 硕士

【摘要】 本文应用RAPD和ISSR技术对凉水国家自然保护区的天然红松(Pinus koraiensis )种群在时间尺度上的遗传多样性变化和遗传分化进行了分析。选择树龄为1—100年的红松个体,每10年为1个龄级,共采集样本245个。10个RAPD随机引物共检测到61个位点, 其中多态位点50个, 多态位点比率为81.97%。 RAPD的Shannon指数龄级间遗传多样性占总多样性的16.43%,RAPD的 Nei指数龄级间遗传分化为13.64%。15个ISSR检测到的位点总数为96个,其中多态位点为84个,种群内多态位点比率为87.50%。ISSR的Shannon指数龄级间遗传变异为18.55%,ISSR的 Nei指数龄级间遗传分化为17.80%。RAPD和ISSR的两种指数都表明,红松在时间尺度上的遗传分化比空间尺度的低,红松的遗传变异主要存在于龄级内。本研究结果证实,凉水保护区内的红松遗传多样性在近100年的时间内出现过大小两次波动,目前该地区的红松遗传多样性正处于上升时期;历史上小规模的采伐虽然暂时降低了红松的遗传多样性,但由于保护区的及时建立,红松的遗传多样性得到了有效的恢复。更多还原

【Abstract】 With RAPD and ISSR method,the paper studied genetic diversity and differentiation in temporal dimension of natural population of Pinus koraiensis in Liang Shui national nature reserve. Total 245 P.Koraiensis samples aged from 1 to 100 years were grouped 10 age classes every 10 years. 61 repeatable loci in which 50 were polymophic were obtained from 10 random RAPD primers and percentage of RAPD polymophic loci was 81.97%.From 15 ISSR primers,96 loci in which 85 were polymophic were obtained and percentage of ISSR polymophic loci was 87.50%. In age class level, genetic differentiation of RAPD Shannon diversity index and Nei index was 16.43% and 13.64%. Genetic differentiation of ISSR Shannon diversity index and Nei index was 18.55% and 17.80%. Two indexes of RAPD and ISSR showed genetic differentiation in temporal dimension is lower than in space and genetic variation existed within age class. Result showed that genetic diversity of P.koraiensis in Liang Shui national nature reserve fluctuated twice in 100 years and is ascending at present. Though genetic diversity of P.koraiensis decreased temporarily after some small fellings, it has restored effectively because of establishment of the nature reserve.更多还原