【作者】 岳静；

【导师】 方宏筠；

【作者基本信息】 辽宁师范大学 ， 植物生化， 2004， 硕士

【摘要】 本论文对PSPC的制备工艺及其生物学活性进行研究。通过多因子的正交试验及对浸提剂种类、浸提剂浓度、料液比、浸提温度、浸提时间的单因素试验得到PSPC最佳提取条件。结果表明:在柠檬酸浓度为0.8%,物料比1:200, 60℃恒温水浴提取2h的条件下可获得PSPC的最大提取量;提取液经旋转蒸发仪浓缩, AB-8大孔树脂吸附法进行纯化,得到色价为225.1的PSPC制备物,具较高的纯度,产率为3.81mg/g薯粉。PSPC制备物抗氧化性、抑菌作用及抗肿瘤试验表明:PSPC具有很强的抗氧化能力,是一种很好的自由基清除剂和抗脂质过氧化抑制剂。PSPC对E.Coli、S.aureus、M.luteus有抑制作用,其抑菌效果与浓度呈正相关;终浓度为1MBCE.Coli(1.25 mg/ml)的PSPC 作用E.Coli,4h后活菌数目即开始下降;6h后,很快进入衰亡期,没有达到正常的生长高峰。PSPC对小鼠肉瘤S180有一定的抑制作用,当抑制剂量为250 mg/(kg·d)和500 mg/(kg·d)时均有显著效果(p < 0.05),抑瘤率达到32.86%,53.12%;且能明显增强患肉瘤S180小鼠血清GSH-Px和SOD的活性,降低小鼠血清中MDA的含量。说明PSPC可通过抗氧化能力而抑制肿瘤的生长。更多还原

【Abstract】 The thesis study on preparation and biological activity of Purple Sweet Potato Color (PSPC). An optimal extractive condition is obtained by the orthogonal experiment of multiplefactor and single factor experiments of the category of extractive reagent , extractive reagent concentration , material rate , extractive temperature , extractive time. The result indicate that the maximum extractive output of PSPC can be gained on the condition of 0.8% citric acid , 1:200 material rate , 60℃ homothermal bainmarie , 2 h. PSPC’s extract were concentrated by rotatory evaporimeter, purified by AB-8 macroporous resin’s adsorption. We can get PSPC’s extract ( E1%1cm532nm ) of higher purity. Its yield is 3.81mg/(g PSPC powder).Study on antioxidative capacity, bacteriostatic action and antitumor effect of PSPC’s extract indicates that PSPC assume a very intensive antioxidative capacity as a better free radical scavenger and anti-lipide’s peroxidation inhibitor. Inhibiting effects on E.Coli、S.aureus、M.luteus of PSPC and its concentration is positive correlation. After the ultimate concentration of 1MBC E.Coli(1.25 mg/ml)PSPC applied 4h to E.Coli , the quantity of viable bacteria began to fall. After 6h, the growth curve didn’t have its peak and turned into degenerative period quickly. PSPC had a given anti-sarcoma S180 capacity. PSPC had conspicuous effect (P < 0.05)when the inhibitory dose were 250 mg/(kg·d) and 500 mg/(kg·d) , inhibition rate run to 32.86%,53.12%. PSPC also can strikingly enhance GSH-Px and SOD activity, decrease MDA’s content in mice plasma. It illustrated that PSPC’s antitumor effect were affect by means of antioxidative capacity.更多还原