【作者】 郑慧芬；

【导师】 何绍江； 王平；

【作者基本信息】 华中农业大学 ， 微生物学， 2004， 硕士

【摘要】 对从饭豆上分离到的根瘤菌进行发光基因gfp-gusA标记，筛选到发光稳定的菌株JMC1402P(带gfp-gusA基因)。JMC1402 P菌落在波长为395nm紫外灯下发绿色荧光。利用这一特点，可以检测JMC1402P在饭豆根圈的定殖密度、结瘤能力及其分布情况。同时利用曲利苯兰(Trypan Blue，TB)染色技术检测丛枝状菌根真菌Glomus mosseae对饭豆的感染率。在无菌有机玻璃盒栽培系统中，研究了不同水分条件下Glomus mosseae菌丝对JMC1402P的垂直迁移作用。在无菌条件下，研究了Glomus mosseae对饭豆植株抗旱，抗重金属汞的作用。 研究结果表明：在无菌土培及沙培条件下，Glomus mosseae提高了JMC1402P的结瘤及固氮效率，而JMC1402P增强Glomus mosseae的侵染率。在无菌有机玻璃盒栽培系统中，JMC1402P能借Glomus mosseae菌丝的延伸作用而垂直散布开来。相对于对照处理，单接种Glomus mosseae和单接种JMC1402P，双接种Glomus mosseae+JMC1402P对饭豆有显著的促生效应。在干旱条件下，接种Glomus mosseae显著改善植株的生长状况，植株对菌根真菌的依赖性随干旱程度的增加而增加。重金属的实验结果表明，Glomus mosseae增强饭豆的抗逆性。更多还原

【Abstract】 The rhizobium JMC1402P containing gfp-gusA genes was obtained by bi-parent matting. The interaction between Glomus mosseae and JMC1402P in rice-legume rhizosphere was investigated in two planting systems. In both systems, there is an positive effect of Glomus mosseae on the population size JMC1402P in the rice-legume rhizosphere. Furthermore, the results of sterile organic glass box planting showed that G.mosseae can transport JMC1402P binding on the hyphae vertically. Compared with CK treatment, JMC1402P can promote the mycorrhizal infectivity of G.mosseae on the rice-bean roots in rhizoxbox-soil microcosms .At the same time, Glmosseae promote the nodulation number of JMC1402P ahead of normal nodulating time. Dual-inoculation oiGlomus mosseae and JMC1402P have a obvious promoting effect on rice-legume growth compared to the treatment of ck. Glomus mosseae also show the obvious positive effect on servivaling of rice bean under rigor soil condition.更多还原