长白山道地中药对小鼠实验性肝损伤的研究

【作者】 崔兴日；

【导师】 南极星；

【作者基本信息】 延边大学 ， 药剂学， 2004， 硕士

【摘要】 提要：目的：探讨长白山道地中药关龙胆、红最天、东当归、东川芎和北柴胡对小鼠急性肝损伤的保护作用。方法：采用D-氨基半乳糖(D-Galactosamine)和内毒素(lipopolysaccharide)造小鼠急性肝损伤模型，观察关龙胆、东当归、北柴胡水提物和醇提物、红景天的水提物和水提醇沉物、东川芎水提物对急性肝损伤小鼠血清中丙氨酸氨基转换酶(ALT)、天门冬氨酸氨基转换酶(AST)的影响，并且观察小鼠肝组织病理改变。结果：腹腔分别注射100mg／kg红景天的水提物和水提醇沉物、东当归的醇提物、东川芎的水提物、关龙胆的水提物及北柴胡的水提物可明显降低因GalN／LPS所致急性肝损伤小鼠血清中丙氨酸氨基转换酶(ALT)、天门冬氨酸氨基转换酶(AST)活力升高。与模型组比较有显著性差异(P＜0.001)。而东当归水提物、关龙胆醇提物和北柴胡醇提物GalN／LPS所致急性肝损伤小鼠血清中氨酸氨基转换酶(ALT)、大门冬氨酸氨基转换酶(AST)活力升高，但模型组比较无显著性差异。(P＞0.05)。病理观察表明红景天的水提物和水提醉沉物、东当归的醇提物、东川芎的水提物、关龙胆的水提物及北柴胡的水提物可减轻因Galn/LPS所致小鼠肝组织损伤。结论：红景天的水提物和水提醇沉物、东当归的醇提物、东川芎的水提物、关龙胆的水提物及北柴胡的水提物对GalN／LPS所致小鼠急性肝损有保护作用。更多还原

【Abstract】 OBJECTIVE: To study the protective effect of Ligusticum acutiloba, Cnidium offcinale, Gentiana manshunca, Bupleurum Chinese, Rhodiola dachaliensis on acute liver injury in mice. METHODS: acute liver injury models induced by D-galactosamine(D-GalN) /Lipopolysaccharid(LPS) in mice, and the levels of serum enzyme ALT, AST on acute liver injury with pretreatment the aqueous and ethanol extract of Gentiana manshunca and Bupleurum Chinese, Rhodiola dachaliensisA, the ethanol extract of Ligusticum acutiloba, the aqueous extract of Cnidium offcinale were observed and we also observed the changes of liver histopathology RESULTS: Pretreatment of mice with the aqueous and etanol extract of Rhodiola dachaliensis, the ethanol extract of Ligusticum acutiloba, the aqueous extract of Cnidium offcinale, Gentiana manshurica, Bupleurum chinese( 100mg/Kg ,intraperittoneal) significantly reduced the levels of serum enzyme ALT, AST on acute liver injury’, and have significant difference compared with control group ( P<0.001) .Althought the aqueous extract of Ligusticum acutiloba, the ethanol extract of Gentiana manshurica, Bupleurum chinese can reduce the levels of serum enzyme ALT. AST on acute liver injury, but the levels of serum enzyme ALT, AST have no significant difference compared with control group (P>0.05) . Histopathologic examination shows that the aqueous and etanol extract of Rhodiola dachaliensis, the ethanol extract of Ligusticum acutiloba, the aqueous extract of Cnidium offcinale, Gentiana manshunca, Bupleurum chinese can relieve liver injury in mice induced by Galn/LPS. CONCLUSION: the aqueous and etanol extract of Rhodiola dachal iensis, the ethanol extract of Ligusticum acutiloba, the aqueous extract of Cnidium offcinale, Gentiana manshunca, Bupleurum Chinese has protective effects for mice acute liver injury induced by D-Galactosamine and lipopolysaccharide.更多还原