【作者】 王洁；

【导师】 童建；

【作者基本信息】 苏州大学 ， 环境卫生， 2004， 硕士

【摘要】 本研究采用双向电泳法分析了不同剂量γ射线辐照前后不同时间大鼠外周血淋巴细胞蛋白质组的改变，在蛋白质水平上研究辐射的生物效应。第一部分采用2Gy全身照射SD大鼠，于照前、照后24h、照后72h分别取样；第二部分采用1、2、4、6Gy照射，于照后24h取样。对样品进行蛋白提取、双向电泳、银染和软件分析，寻找差异表达的蛋白质。 发现了辐照前后不同时间有21个差异表达的蛋白点，其中7个蛋白点在辐照后表达消失；4个在照后24h表达减弱、照后72h表达消失；4个蛋白点在辐照后24h、72h表达减弱；6个蛋白点在照后24h、72h表达增强。不同剂量照射后共有17个差异表达蛋白点，其中2个蛋白点在各辐照组均表达消失；2个在各辐照组表达均增强；5个在各辐照组表达均减弱；其余蛋白点在各辐照组随辐照剂量的增加逐渐减弱至消失。研究结果表明电离辐射能够诱导大鼠外周血淋巴细胞蛋白质组表达的改变，包括蛋白表达的消失，和表达量的增加或减少；γ射线照射后不同时间，某些蛋白的表达量显示出一定的时间-效应关系；不同剂量γ射线照射后，某些蛋白的表达量具有一定的剂量-效应关系。这些差异蛋白可能和辐射损伤以及辐射诱导的应激反应有关。更多还原

【Abstract】 To elucidate the radiation effect on the proteome of rat peripheral blood lymphocytes, the proteome levels before and after ionizing radiate were compared by the technic of two-dimensional electrophoresis (2-DE). The proteir levels were measured at different times of 24h and 72h after the 2Gy total-body irradiation. The effect of different doses were also analysed for 1, 2, 4 and 6 Gy total-body irradiation. The total protein samples prepared by one-step method were separated by the h: gh resolution 2-DE and silver stain, and the resulted spots were detected by the Imagemaster 2DE software.Seven protein spots disappeared and sixteen protein spots showed significant differences in quantity at 24h and 72h after the 2Gy total-body irradiation. In the 1, 2, 4 and 6Gy irradiation groups, two protein spots disappeared, Two other spots was up-regulated and thirteen spots were down-regulated vdth increase of the irradiation doses. The results showd reveals that ionizing radiation could induce significant changes in protein expression of rat peripheral blood lymphocytes. The time-effect and dose-effect relations could be induced for the expression of some protein spots upon irradiation, which might be a reflection of the response to the irradiation damages.更多还原