【作者】 李丹；

【导师】 刘进；

【作者基本信息】 辽宁中医学院 ， 中医基础理论， 2004， 硕士

【摘要】 脾阴虚证是脾的阴液亏虚，运化失常，精微无源，散精不足的病理变化，表现为饥不欲食，口唇干燥，大便秘结，形体消瘦，舌红少苔等。目前对脾的研究已深入到分子水平，现发现脾虚证与信号转导的关系十分密切。在多条信号通路中，MAPK信号转导通路是多种信号转导途径的共同作用部位。 有丝分裂原激活的蛋白激酶(Mitogen Activated Protein Kinase，MAPK)是一组分布于胞浆中、具有丝氨酸和酪氨酸双重磷酸化能力的蛋白激酶，是丝／苏氨酸激酶。迄今已证明真核细胞中MAPK家族有四个成员。MAPK广泛分布于胞浆，也分布在核区。在细胞受有丝分裂原激活后，胞质内合成的MAPK可转到核区，通过磷酸化多种底物来调节细胞生理过程。凡是兴奋G蛋白耦联受体的物质(如血管紧张素、加压素、儿茶酚胺和内皮素等)和激活酪氨酸激酶的物质(如生长因子和其它细胞因子等)刺激细胞分裂时，都要通过MAPK激活。MAPK信号转导通路采用高度保守的三级激酶级联反应传递信号。MAPK被激活后可以移至细胞核内，通过磷酸化转录因子、细胞骨架相关蛋白和其它酶类等多种蛋白底物而参与细胞生长发育、增殖、分裂、凋亡及应激反应等多种细胞功能的调控。 目的：本项研究旨在通过观察脾阴虚证大鼠模型MAPK活性变化，探讨在脾阴虚状态下大鼠脑、肝、心组织MAPK活性变化规律及其调控机制，进一步揭示脾阴虚证的病理实质和滋脾阴法的作用，从而为脾实质的研究提供客观依据。 方法：根据中医学的病因发病学理论，本项研究采用饮食不节、疲劳过度和药物损伤等综合因素，成功建立脾阴虚证大鼠模型，在此基础上对治疗组应用滋脾阴方药进行治疗，与脾阴虚证模型组、正常对照组对比。实验第90天，对所有大鼠禁食24小时后，施行20％乌拉坦腹腔注射麻醉，断头取脑、心、肝，置于-70℃低温冰箱中保存备检。 MAPK活性测定：免疫沉淀法。 结果： 1．脾阴虚证大鼠肝脏、心脏、脑组织的MAPK活性变化的比较 脾阴虚证大鼠心脏组织的细胞浆MAPK活性有明显升高：肝脏组织、脑组织的细胞浆MAPK活性虽有变化趋势，但无统计学意义。 2．滋脾阴法对脾阴虚证大鼠肝脏、心脏、脑组织的MAPK活性变化的影响 滋脾阴方药能明显升高脾阴虚证治疗组大鼠心脏组织的细胞浆MAPK活性，并使其显著高于正常水平。滋脾阴方药对脾阴虚证治疗组大鼠肝脏、脑组织的细胞浆MAPK活性虽有影响，但无统计学意义。 结论：脾阴虚证大鼠心脏、肝脏、脑组织的MAPK活性发生变化，滋脾阴方药能影响MAPK信号转导通路。更多还原

【Abstract】 Objective: To investigate the changes of activities of mitogen-activated protein kinase(MAPK) in the liver, heart, brain of the rats with spleen-yin deficiency syndrome and the effective mechanism of Chinese herbs for replenishing the spleen yin.Methods: The rat model of spleen-yin deficiency was set up by applying the multiply factors of improper diet, overstrain and damage from abuse administration drugs. And the activities of MAPK in the liver, heart, brain tissue were detected by substrate phosphorylation method.Results: (1)The MAPK activity in the heart tissue of rats with spleen-yin deficiency was increased obviously. The MAPK activity in the liver has the increasing tendency but has the decreasing tendency in brain tissue. (2) The herb of replenishing the spleen-yin could increase the MAPK activity of the cytoplasm of the heart tissue of the rats with spleen-yin deficiency, but there was little influence on the MAPK activity in the brain and heart tissue.Conclusions: It was shown that the MAPK activities of the liver, brain and heart tissue changed in the rats with spleen-yin deficiency syndrome, and the herbs of replenishing the spleen-yin had effect on the MAPK signal transduction pathway.更多还原