【作者】 罗娅；

【导师】 汤浩茹；

【作者基本信息】 四川农业大学 ， 果树学， 2004， 硕士

【摘要】 矮化密植是当今果树发展的总趋势。几乎所有梨都嫁接在砧木上，因此对优良砧木的需求是非常紧迫的。本研究以我国研制的优良梨矮化砧木‘S₂’、‘S₅’、‘PDR₅₄’和栽培品种‘身不知’的茎尖为外植体，通过正交试验设计并结合方差分析和多重比较，比较栽培品种与砧木品种在增殖和生根培养的差异并探讨了四品种最佳增殖和生根培养基配方，为规模化生产梨矮化自根砧苗木和提高繁育速度奠定一定的理论和技术基础。其试验结果表明： （1） 继代增殖试验中通过增殖倍数、平均株鲜重、平均株干重和平均株苗高度差分析表明四个品种的增殖培养基分别是： ‘身不知’：MS+1.0mg／LBA： ‘S₂’：MS+3.0mg／LBA+0.2mg／LIBA+2.0mg／LGA₃； ‘S₅’：MS+2.0mg／LBA+1.0mg／LIBA+1.0mg／LGA₃； ‘PDR₅₄’：QL+3.0mg／LBA+0.1mg／LIBA+4.0mg／L GA₃。 （2） 结合生根率、平均根数、平均根长、平均株根鲜重及平均株根干重全面分析，四个品种的最佳生根条件分别是： ‘身不知’：在MS+2.5mg／LIBA+5.0g／L蔗糖上暗培养10天后转入MS+2.5mg／LIBA+5.0g／L蔗糖+1.0g／LAC； ‘S₂’：在1／2QL+5.0 mg／LIBA+5.0g／L蔗糖上暗培养10天后转入1／2QL+5.0 g／L蔗糖+0.5 g／LAC； ‘S₅’：在1／2MS+5.0mg／LIBA+15.0 g／l蔗糖上暗培养10天后转入1／2MS+15.0 g／L蔗糖+1.0g／LAC； ‘PDR₅₄’：在1／4MS+2.5 mg／LIBA+15.0g／L蔗糖上暗培养10天后转入1／4MS+2.5 mg／LIBA+15.0g／L蔗糖+1.0g／LAC。更多还原

【Abstract】 In this study the shoot tips isolated from P. pyrifolia ’ Shehbuzhi’, ’S2’, ’S5’ and ’PDR54’ were used as explants. By means of the orthogonal experiment design, analysis of variance and multiple comparisons, the factors including genotupes, basical medial, plant growth regulators were analysed. The results were as follow.-(1) Considering the comprehensive index shoot qulity numbers and length, the best sub-culture medium for in vitro perliferation of four varieties was respectively as follows:For ’Shenbuzhi’ , the sutiable prescription for proliferation was MS + 1.0mg/LBA.For ’ S2’, the best medium of proliferation was MS+3mg/LBA+0.2mg/LIBA +2mg/LGA3.For ’S5’, the sutiable prescription for proliferation was MS+2.0mg/LBA+ lmg/LIBA+lmg/LGA3.For ’PDR54’, the best medium of proliferation was QL + 3.0mg/LBA+ 0.1mg/LIB A+4mg/L GA3.( 2 ) According to rooting rate, mean root number, mean root length, root fresh weight per shoot and root dry weight per shot tested, the optimal rooting condition for four viarieties was respctively as follows:For ’ Shenbuzhi’, microcuttings were incubated the root iniation medium 1/4MS supplemented with IB A at 5.0mg/LIBA and sucrose at 30.0g/L in darkness for 10 days, followed by 1/4MS containing sucrose at 30.0g/L and 1.0g/LAC.For ’ S2’, shoots were placed in the medium of 1/2QL supplemented with IBA at 5.0mg/LIBA and sucrose at 5.0g/L in darkness for 10 days, followed by 1/2QL containing sucrose at 5.0g/L and 0.5g/LAC.For ’ S5’, microcutting were inoculated in 1/2MS containing IBA at 5.0mg/L and sucrose at 15.0g/L in darkness for 10 days, and then transferred into root formation medium 1/2MS supplemented with IBA at 5.0mg/L , sucrose at 15.0g/L and 0.5g/LAC.For ’PDR54’, shoots were first inoculated in 1/4MS containing IBA at 2.5mg/LIBA and sucrose at 15.0g/L in darkness for 10 days, and 1/4MS supplemented with IBA at 2.5mg/L, sucrose at 15.0g/L and 1.0g/LAC for the rest.更多还原