【作者】 任晓燕；

【导师】 张玲敏；

【作者基本信息】 暨南大学 ， 病原生物学， 2004， 硕士

【摘要】 目的： 研究α-三噻吩对白纹伊蚊幼虫的毒杀作用及其组织学的改变。 方法： 1．在近紫外光（UVA）下，确定黑暗中不同处理时间与α-三噻吩（α-T）对白纹伊蚊幼虫毒杀作用的关系。 2．测定UVA下，α-T对白纹伊蚊各期幼虫的毒杀作用。 3．观察白纹伊蚊幼虫吸食α-T并受UVA照射后生长发育的改变。 4．日光下，α-T对白纹伊蚊幼虫的毒杀作用。 5．光学显微镜下观察α-T作用后白纹伊蚊幼虫组织学的改变。 6．扫描电镜和透射电镜下观察α-T作用后白纹伊蚊幼虫中肠和马氏管的超微结构的改变。 结果： 1．黑暗中处理时间为3h时，α-T对白纹伊蚊幼虫的毒杀作用最大。 2．在UVA下，α-T对白纹伊蚊4龄幼虫的LC₅₀为2.37μg／L；而且龄期越小，敏感性越高。 3．α-T能降低白纹伊蚊幼虫的化蛹率、羽化率，延长平均化蛹时间、平均羽化时间。 4．在日光下观察，于5 am施药对白纹伊蚊幼虫的毒杀作用显著优于10 am和1 pm施药的毒杀作用；在强烈阳光辐射下α-T可以快速杀灭白纹伊蚊幼虫。 5．在光镜下观察，α-T对白纹伊蚊幼虫的靶标是中肠、马氏管和脂肪体。 6．在扫描电镜下观察，α-T可使白纹伊蚊幼虫中肠微绒毛分布不均、稀少、凌乱。 7．在透射电镜下观察，白纹伊蚊幼虫中肠细胞胞质密度降低，线粒体、内质网肿胀；马氏管细胞内小型结晶体增多，结晶体内黑色物质增多。a一三唾吩对白纹伊蚊幼虫毒杀作用的研究中文摘要结论:1 .Q一T具有光活化特性，对白纹伊蚊幼虫有高效的毒杀作用，并能抑制其生长 发育。2.组织学研究证实，a一T在组织水平上主要作用于中肠、马氏管和脂肪体，在 细胞水平上主要作用于生物膜系统，触杀、胃毒作用是其主要作用方式。更多还原

【Abstract】 Objective:To study the toxicity and histomorphological changes of Aedes albopictus larvae by alpha-terthienyl.Methods:1. To identify the relationship between different treating time in dark and the toxicity of alpha-terthienyl under UVA to Aedes albopictus larvae.2. To determine the toxicity of alpha-terthienyl to different instars of Aedes albopictus larvae under UVA.3. To determine the effect of alpha-terthienyl on the growth of Aedes albopictus larvae under UVA.4. To investigate the toxicity of alpha-terthienyl to Aedes albopictus larvae under sunlight.5. To observe the histopathological changes of Aedes albopictus larvae by light microscope.6. To observe the ultrastructure changes of Aedes albopictus larvae in midgut microvilli, midgut cell, malpighian tubule with electron microscopes(SEM and TEM).Results:1.The most effective toxicity of alpha-terthienyl to mosquito larvae occurred as the treating time in dark was 3 hours.2. Under UVA and alpha-terthienyl, median lethal concentration (LC50 ) to Aedes albopictus larvae of 4th instar was 2.37#g/L; the smaller the larvae instar were , the more suspectibility larvae were.3. Alpha-terthienyl could reduce the pupation and emergence, prolonged the averagetime of pupation and emergence.4. Mosquito larvae could be quickly killed under strong sunlight radiation; the effect of administing alpha-terthienyl at Sam was better than that at 10am and 1pm.5. The observation with light microscope showed that the targets of alpha-terthienyl to Aedes albopictus larvae were midgut, malpighian tubule and fat body.6. The observation with scanning electron microscope showed that microvilli became fewer and fallen disordered partly absent from the cells.7. The observation with transmission electron microscope approved that the decreased cytoplasm density and swelled mitochondria and endoplasmic reticulum in midintestine cells; the smaller concretions in the cytoplasms of malpighian tubules increased , the black substance in the concretions increased.Conclusion:1. Alpha-terthienyl had light-activated, high effect to kill Aedes albopictus larvae, It could inhibit the growth of mosquito.2. The results of histology study showed that the tissular targets of alpha-terthienyl were midgut malpighian tubule fat body and the cellular targets were biological membrane system; contact action and stomach action were the important insecticidal modes of alpha-terthienyl.更多还原