【作者】 张咏梅；

【导师】 陈以旺；

【作者基本信息】 福建医科大学 ， 药理学， 2004， 硕士

【摘要】 【目的】 研究竹醋液中的铁螯合剂落叶松皮素对细菌生物膜发育及生物膜菌耐药性的影响,并对其作用机制进行初步探索,从而为运用外源性铁螯合剂防治细菌生物膜相关感染及竹醋液在医药上的应用提供理论依据。 【方法】 建立铜绿假单胞菌生物膜体外静置模型,检测落叶松皮素对铜绿假单胞菌黏附性、IV型菌毛颤搐运动和该菌生物膜胞外多糖蛋白复合物含量的影响;测定落叶松皮素处理与否,磷霉素对铜绿假单胞菌生物膜菌杀灭效果的差异。 【结果】落叶松皮素使铜绿假单胞菌对塑料表面的黏附性下降(P=0.001),其抗黏附作用随着浓度的升高而增强。落叶松皮素处理的铜绿假单胞菌生物膜胞外多糖蛋白复合物含量无明显变化(P=0.227)。落叶松皮素可促进铜绿假单胞菌IV型菌毛颤搐运动(P=0.000),随着浓度升高,IV型菌毛颤搐运动加强。落叶松皮素可增加磷霉素对铜绿假单胞菌生物膜菌的杀灭效果(P=0.000),存活菌落数对数降低值随着后者浓度的增加、作用时间的延长呈现出上升的趋势。 【结论】 铁螯合剂落叶松皮素通过促进铜绿假单胞菌IV型菌毛颤搐运动,影响该菌生物膜的发育,同时在某种程度上可减弱生物膜菌对磷霉素的耐药性。运用外源性铁螯合剂,可能是清除细菌生物膜,防治细菌生物膜相关感染的一种新策略;竹醋液可能有用于防治细菌生物膜相关感染的潜力。更多还原

【Abstract】 【Objective】To investigate the effects of iron chelator maltol on the development of Pseudomonas aeruginosa biofilm and biofilm bacterial antibiotic resistance; to explore a new approach in the prevention of biofilm bacterium-associated infections; and to provide a theoretical base for the use of bamboo vinegar in medicine.【Methods】A static Pseudomonas aeruginosa biofilm model was developed to investigate the effect of iron chelator maltol on 1) the biofilm-producing Pseudomonas aeruginosa’s surface adherence, 2) the production of glycocalyx in Pseudomonas aeruginosa biofilm,3) the twitching motility of Pseudomonas aeruginosa ,and 4) Pseudomonas aeruginosa’s susceptibilty to fosfomycin.【Results】⒈Pseudomonas aeruginosa’s surface adherence was decreased with increasing maltol concentration. There was significant difference between control and treatment, and significant difference among the three maltol concentrations tested(P=0.001).⒉Maltol had no effect on the production of glycocalyx in Pseudomonas aeruginosa biofilm.There was no significant difference between the control and treatment(P=0.227).⒊By chelating iron, maltol stimulated twitching motility of Pseudomonas aerugino., There was a significant difference between control and treatment, and significant difference among the three maltol concentrations tested(P=0.000).⒋In combination with maltol, fosfomycin killed biofilm-producing Pseudomonas aeruginosa efficiently. There was a significant difference between control and <WP=5>treatment(P=0.000).【Conclusions】 Iron chelator maltol affects the development of Pseudomonas aeruginosa biofilm and can decrease biofilm bacterial resistance to fosfomycin to some extent. Our date suggest that iron chelator and bamboo vinegar should be investigated further as a possible therapeutic agents against biofilm bacterium-associated infections.更多还原