【作者】 张学颖；

【导师】 段舜山；

【作者基本信息】 暨南大学 ， 水生生物学， 2004， 硕士

【摘要】 研究以蛋白核小球藻和杜氏盐藻为材料，盐藻设定7个添加Cr³⁺浓度处理，小球藻设定8个添加Cr³⁺浓度处理，测定了不同Cr³⁺浓度下微藻的生物量（细胞密度）、微藻对Cr³⁺的富集量、叶绿素a、β-胡萝卜素、蛋白质及可溶性糖含量；选择其中对微藻生长和富集较优的添加Cr³⁺浓度，比较了Cr³⁺的不同添加方式对微藻生物量及富集量的影响；进行了两种微藻的三因素三水平正交试验，测定了不同组合的生物量和富集量；并探讨了最佳组合条件下的Cr³⁺富集动态过程；分析了生物富集对微藻细胞分子官能团结构的影响。结果表明，培养液的Cr³⁺浓度为1.0mg·L^-1时，可以提高小球藻的生物量、叶绿素a、蛋白质和可溶性糖的含量。小球藻富集Cr³⁺的适宜添加条件为：在接种第1天至第5天期间分批次添加Cr³⁺，其最终加入是1.0mg·L^-1，收获时测小球藻的生物量及富集量分别是2.25mg·mL^-1和82.89μg·g^-1，小球藻对Cr³⁺的富集效率为88.7％，与一次性添加相比较，小球藻的富集量提高了28.2％。小球藻获得最大生物量和富集量的优化组合为：温度30℃，pH7，N3.0g·L^-1，P0.08g·L^-1，此时的生物量为2.38mg·mL^-1，富集量为85.35μg·g^-1。Cr³⁺浓度为0.2mg·L^-1时，可以提高盐藻的生物量、β-胡萝卜素、蛋白质、可溶性糖含量。盐藻富集Cr³⁺的适宜添加条件为：在接种第1天至第8天期间分批次添加Cr³⁺，其最终加入是0.2mg·L^-1，收获时测盐藻的生物量及富集量分别是0.93mg·mL^-1和40.23μg·g^-1，盐藻对Cr³⁺的富集效率为90.6％，与一次性添加相比较，盐藻的富集量提高了32.6％。盐藻获得最大生物量和富集量的优化组合为：温度30℃，pH7，盐度70‰，此时的生物量为0.98mg·mL^-1，富集量为42.77μg·g^-1。更多还原

【Abstract】 In this paper, we used Chlorella pyrenoides and Dunaliella salina as experimental material to assess the optimal cultivation conditions of Cr enrichment by the two alga species through cultivation experiments by Cr3+ addition in the medium. Chlorella pyrenoides was cultivated under the 7 different concentrations of Cr3+ and Dunaliella salina was cultivated under the 8 different concentrations of Cr3+. We measured biomass (cell density) of the two algae, the amount of Cr3+ enrichment, contents of chlorophyll-a, β -carotene, intracellular protein and carbohydrate in the different Cr3+ addition treatments. Based on these experiments, we used orthogonal experiment with three factors and three levels, analyzed the effects of different methods of Cr3+ addition on algal biomass and Cr enrichment and discussed the dynamic processes of Cr3+ enrichment under the optimal combination conditions. In addition, the effects of Cr3+ enrichment on the algal cell molecular functional group structure were also investigated. The result of this study indicated that Cr3+ significantly increased the biomass of Chlorella pyrenoides and its contents of chlorophyll-a, β -carotene, protein and carbohydrate at the Cr3+ concentration of Img ?L"-1. The optimal concentration of Cr3+ was Img ?L-1 that should be added day by day to the medium during the initial 5d of culture. At this condition, harvested biomass and Cr enrichment quantity of Chlorella pyrenoides were 2.25mg ?mL-1 and 82.89 *g ?g-1, respectively, with an enrichment efficiency of 88.7%. The optimal combination of temperature, pH and N, P concentration depended on the experimental intention. To improve the growth and Cr3+ enrichment of Chlorella pyrenoides, the optimal combination of temperature, pH and N, P concentration is 30 ℃ in temperature, 7 in pH, 3g ?L-1 in N concentration and .08g ’L-1 in P concentration, with the highest biomass of 2.38mg ’mL-1 and the highest enrichment quantity of 85.35 * g ?g-1. The concentration of 0.2mg ?L-1 in Cr3+ can significantly increased the biomass of Dunaliella salina and its contents of chlorophyll-a, 3 -carotene, protein and carbohydrate. The optimal concentration of Cr3+ was 0.2mg ?L-1 that should be added day by day to the medium during the initial 8d of culture. At this condition, harvested biomass and Cr enrichment quantity of Dunaliella salina were 0.93mg ?mL-1 and 40.23 * g ?g-1, respectively, with an enrichment efficiency of 90.6%. The optimal combination is 30℃ in temperature, 7 in pH and 70 %o in salinity because the highest biomass of 0.98mg ?mL-1 and the highest enrichment quantity of 42.77 μ g ?g-1.更多还原