【作者】 张文慧；

【导师】 杨庆凯； 李文滨； 陈庆山；

【作者基本信息】 东北农业大学 ， 作物遗传育种， 2004， 硕士

【摘要】 大豆灰斑病（Cercospora sojina Hara）是世界性病害，也是我国东北大豆主要病害之一，对大豆的产量和品质的影响十分严重，而防治大豆灰斑病最为经济有效的方法就是种植抗病品种，因此，抗灰斑病育种工作倍受重视。要使品种的抗病性在生产上保持更长时间，就需要培育抗多个生理小种的品种，这就需要对抗病基因进行标记与定位。 本实验针对中国大豆灰斑病1号生理小种，以抗所有生理小种的品系东农40566为母本，以感1号生理小种的品种东农410为父本配制杂交组合，杂交得到F₂代后连续自交3代得到F₅代群体。该群体经人工接种灰斑病1号生理小种后进行抗性鉴定，结果表明抗病株系和感病株系符合1∶1分离规律，说明大豆对灰斑病1号生理小种的抗性是由单一显性基因控制的。利用BSA法对500个SSR标记进行筛选，其中3个标记Satt565、SOYGPATR和Satt396在抗、感池间表现出稳定的多态性，并且在F₂代个体中表现出抗性与多态性协同分离的趋势。这3个标记与抗性基因的连锁顺序为Satt565—SOYGPATR—Hrcsl—Satt396，它们与抗性基因的连锁距离分别为12.7cM、6.5cM、14.7cM。推测抗大豆灰斑病1号生理小种的基因可能位于C1连锁群上。 将鉴定出的与抗性基因有连锁关系的三个SSR标记与黑龙江省的45份抗感种质资源进行符合性检测，并对三个SSR标记的辅助鉴定效率进行分析，结果表明：SOYGPATR-2和SOYGPATR-3鉴定抗病资源的效率较高，可分别达到81.8％和83.3％，Satt396-1鉴定抗病资源的效率可达72.7％。这三个等位变异对抗性资源的筛选有一定的参考价值。更多还原

【Abstract】 Cercospora sojina Hara occurs worldwide and is one of major soybean disease in northeast of China, harming soybean seriously. The most effective and economical way is planting resistant cultivars, so, breeding resistant cultivar is important by finding resistant gene location, which with long-time and more -races resistance to Cercospora sojina Hara.In aim to find the SSR markers linked to resistance gene against frogeye leaf spot in Chinese soybean, Bulked segregant analysis was applied to F5 populations derived from cross between the resistant line DongNong40566 and the susceptible line DongNong410, self-crossing for three generations after F2. The resistant and susceptible proportion show that Cercospora sojina Hara was resistant by single gene . three SSR markers including Satt565, SOYGPATR and Satt396 were identified from 500 SSR markers, the linkage order is Satt565 SOYGPATRHrcslSatt396, and the distance to the resistance locus is 12.7,6.5> 14.7cM respectively. These three markers and the resistance locus were mapped to soybean linkage group C1.Three SSR Markers Satt565, SOYGPATR and Satt396 were identified by 45 resistant and susceptible lines, result show that allele SOYGPATR-2, SOYGPATR-3 and Satt396-l accord with resistant and susceptible trait highly. They can identify resistant cultivars with81.8%> 85.7% > 72.7% probability. So, they have some value in resistance source screening.更多还原