【作者】 朱国英；

【导师】 郝再彬；

【作者基本信息】 东北农业大学 ， 植物学， 2004， 硕士

【摘要】 目前，东北大豆遗传基础狭窄已经成为大豆产量进展缓慢的主要原因之一。拓宽大豆的遗传基础，利用和创造新种质是使大豆产量取得突破的根本途径。利用化学诱变剂进行诱变突变率高且简单易行，是创造新种质的有效途径。经化学诱变剂处理的材料品质发生很大变异。本实验检测了经NaN3处理的东农42和东农163产生的86个品系及16个黑龙江主栽品种的大豆异黄酮、醇溶蛋白、水溶蛋白、碱溶蛋白、油分及脂肪酸组分的含量。结果表明，NaN3诱变的品系变异丰富。首先，在大豆异黄酮方面，组1（来源于东农42的突变体品系）中，87.5%的品系大豆异黄酮含量极显著低于对照，组2（来源于东农163的突变体品系）中大豆异黄酮含量极显著低于对照和极显著高于对照的各占46.3%；其次，在醇溶蛋白含量方面，组1各品系醇溶蛋白含量均高于对照且差异极显著，组2的各品系中40.7%极显著高于对照，9.3%品系显著低于对照；在水溶蛋白含量方面，组1各品系中水溶蛋白含量极显著高于对照的占9.38%，极显著低于对照的占78.12%，组2各品系中,它们分别是35.19%和46.30%；在碱溶蛋白含量方面，组1各品系中只有1个品系碱溶蛋白含量极显著低于对照，而在组2各品系中，碱溶蛋白含量极显著高于对照的占25.93%，极显著低于对照的占40.74%；最后，在油分含量方面，组1各品系油分含量均高于对照，且25%差异极显著，组2各品系中25.9%极显著高于对照，59.3%极显著低于对照。通过检测，筛选出了与对照差异极显著的品系，同时选出了一些在某一方面含量高于或低于所选全部农业推广品种的品系。 油分总量与软脂酸、硬脂酸、亚油酸含量呈极显著的负相关，与油酸含量呈极显著正相关。从来源于东农42各品系中筛选出4个品系油酸或亚油酸含量高于对照同时亚麻酸含量又低于对照的品系，从来源于东农163各品系中筛选出了5个这样的品系。更多还原

【Abstract】 The stricture of genetic ground in northeastern soybean results in the yield of soybean increase slowly.Creasing and making use of new germplasm resource to widen the genetic ground is the basic approach to increasing yield of soybean.Taking advantage of chemical inducer is a effect approach,it can be practiced easily and the ratio of mutant is high.The variation of resource is great by treatment with NaN3 , 86 breed derived Dongnong42 and dongnong 163 and 16 agricultural generalized breed are tested in this research on the quantity of oil,fatty acid,prolaminand, isoflavone. the results show that on the quantity of isoflavone,87.5%of the breed are higher than contrast very obviously in group 1,and in group 2 the percentage of higher and lower are both 46.3%; on the quantity of prolaminand, all of the breed are higher than contrast very obviously in group 1, and in group 2 the percentage of higher and lower are 40.7% and 9.3%; on the quantity of watersolved protein,9.38% of the breed are higher than contrast very obviously and 78.12% are lower obviously in group 1, and in group 2 the percentage of higher and lower are35.19% and 46.30%. on the quantity of basicsolved protein,1 breed is lower than contrast very obviously,and in group 2 the percentage of higher and lower are 25.93% and 40.74%;on the quantity of oil , 25%of the breed are higher than contrast very obviously in group 1, and in group 2 the percentage of higher and lower are 25.9% and 59.3% ,we select the species varied obviously .the breed that differ from contrast very obviously and some of their quantity beyond all the agricultural generalized breed are selected.The determination of fatty acid component show that the quantity of fatty acid is reletive to quantity of oil, and we select the 9 breeds which quantity of linolenic acid is lower than contrast .更多还原