杜仲叶中绿原酸的提取及动力学研究

【作者】 李进飞；

【导师】 黄可龙；

【作者基本信息】 中南大学 ， 应用化学， 2004， 硕士

【摘要】 杜仲叶为杜仲科杜仲属植物杜仲(Eucommia Ulmoides Oliv．)的叶子。近年来研究表明，杜仲叶与杜仲皮的有效成分基本相同，甚至某些有效成分(如绿原酸)的含量远远高于皮。绿原酸(CGA)是杜仲叶中含量较高的有效成分之一，具有多种重要的药理作用，尤其具有良好的抗恶性肿瘤作用。因此，从丰富的杜仲叶资源中提取分离出较高含量的绿原酸提取物，具有重要的意义。 本文在详细总结了目前杜仲叶中绿原酸提取与分离研究的基础上，对用乙醇溶液提取和大孔吸附树脂分离法进行了深入研究。通过正交实验和单因素实验对提取溶剂、提取时间、料液比、提取温度和提取液pH进行考察，确定了最佳提取工艺。在对绿原酸的分离富集时，采用了对环境和人体无毒害作用的树脂吸附工艺，通过对四种不同大孔吸附树脂对绿原酸的吸附分离性能的研究，筛选出NKA-9型树脂用于杜仲叶提取液中绿原酸的分离富集，进一步考察了该树脂的动态吸附性能，进样液pH值、进样液浓度、进样液流速对树脂吸附性能的影响，以及洗脱剂的选择，得出最佳分离条件。 在理论研究上，基于超声辅助提取技术在中药有效成分的提取中具有独特的优点，本文在修正后的Fick扩散第一定律基础上，结合超声场对固液扩散机制的影响，研究了超声场强化中药提取时，中药有效成分提取过程的动力学方程，并同常规浸提法的动力学方程比较，对强化机理进行了一定的探讨，并用试验数据对该方程进行检验，得出超声辅助中药提取的动力学方程，对超声辅助中药有效成分提取实验提供了一定的理论参考。更多还原

【Abstract】 The leaves of Eucommia Ulmoides Oliv. have been studied a lot recently. It has been found that the leaves have almost the same effective components as the bark. Some components are even’ more in the leaves than in the bark such as chlorogenic acid. Chlorogenic acid is one of the main effective components. It plays many important pharmacological roles especially anticarcinogenicity and antigenotoxicity. So it has economic signification that extraction and purification of chlorogenic acid from abundant leaves of Eucommia Ulmoides Oliv.In this paper, extraction and separation of chlorogenic acid from the leaves of Eucommia Ulmoides Oliv. were reviewed. Alcohol-extractive and then resin adsorption method was further studied. Through studying the effects of extractive solution, extractive time, ratio of solvent to material, extractive temperature and pH value of the solution on the yield of Chlorogenic acid, the optimum condition are determined. Resin adsorption method was used to enrich and isolation chlorogenic acid from the extraction solution. By comparing four different macroporous resins, NKA-9 was selected to separate chlorogenic acid. In order to find out the optimum conditions, the factors of effecting adsorption and elution were also researched, such as pH value, concentration, speed of adsorption, amount of resin, effluent speed, concentration and eluting agent.Assisted-ultrasonic extraction has been widely used in the effective components extraction of Chinese traditional medicine for its peculiarity. A kinetic equation, for assisted-ultrasonic extraction process was presented based on the revised Fick’s first law of diffusion and ultrasonic enhancement of solid-liquid diffusion. Experiment data were studied to evaluate the kinetic equation. The experiment data curve can almost match with the kinetic equation completely. So it provides a theoretical reference for the assisted-ultrasonic extraction of Chinese traditionalmedicine.更多还原