【作者】 曾绍校；

【导师】 郑宝东；

【作者基本信息】 福建农林大学 ， 农产品加工及贮藏工程， 2004， 硕士

【摘要】 为了开发福建余甘资源，对余甘中多糖的最佳提取工艺进行了研究，并按保健食品功能学评价程序对余甘多糖的抗氧化、抗肿瘤作用进行研究。 在单因素试验的基础上，通过正交实验确定了余甘多糖提取最佳工艺为采用品种为南大、100℃下浸提4h，提取率达到1.18％。 通过观察余甘多糖对CCl₄肝损伤小鼠的影响来研究其抗氧化作用，结果表明余甘多糖能显著降低小鼠肝脏中过氧化脂质（LPO）含量，并显著提高全血中超氧化物岐化酶（SOD）活性和谷胱甘肽（GSH）的浓度（P＜0.05），说明余甘多糖具有抗氧化作用，提示其可能有助于延缓衰老。 余甘多糖对S₁₈₀腹水瘤体外初步实验观察表明，余甘多糖各剂量能导致癌细胞破裂，细胞空泡，核固缩，说明余甘多糖可能具有抗肿瘤作用。 以环磷酰胺（CTX）为阳性对照组，以生理盐水为阴性对照组，观察各剂量余甘多糖对荷瘤(腹水瘤S₁₈₀和H₂₂)小鼠生存天数的影响，结果表明高、中剂量的余甘多糖能显著延长荷瘤(S₁₈₀)小鼠的生存天数（P＜0.05），中剂量的余甘多糖能显著延长荷瘤(H₂₂)小鼠的生存天数（P＜0.05）；采用小鼠体内淋巴细胞转化实验和小鼠碳廓清实验来观察余甘多糖对荷瘤(实体瘤S₁₈₀)小鼠免疫功能的影响。结果表明，高、中、低剂量的余甘多糖对小鼠肉瘤均有抑制作用，抑瘤率分别达到52.13％、50.08％、31.33％：而中、低剂量的余甘多糖对小鼠的免疫功能没有抑制作用。余甘多糖的抑制肿瘤的机理可能是通过增强肌体免疫力和抗氧化作用来干预癌细胞的生长实现的。更多还原

【Abstract】 For the sake of exploiting fujian Phyllanthus Emblica L. resources, the extraction technology of polysaccharide from Phyllanthus Emblica L. (PePs) and its functional effect of antioxygenation and antitumor was studied according to functional evaluation process of health food.On the basis of single factor experiments, the optimum extraction technology was determined through orthogonal experiments: extracting 4h under the temperature of 100C by use of N AND A.The effect of PePs on carbon tetrachloride intoxicated mice was studied to investigate its antioxidation. Results indicated that PePs obviously reduced LPO (Lipid peroxidation) contents in intoxicated mice liver, enhanced the SOD activity and the GSH contents in the blood(P<0.05). It is proved that PePs has the effect of antioxidation, and thus delaying senescence possibly.Preliminary experiment of antitumor(S180) in vitro showed that PePs can break the cancer cell and make the cell vacuolus and karyopycnosis which indicated that PePs had possibly antineoplastic activity.Model groups(transplanted S180 and H22 model in mice), control groups(CTX and physiological saline) and treated groups(4g/L 3g/L 2g/L 1g/L PePs solution) to abserve the antineoplastic activity and the effect of immune functions noduliferous mice by calculating the lymphocyte convertion rate and phagocytosis index. The results showed that high dose and middle dose can prolong remarkably the survival time of mice planted with S180 fluid tumor(P<0.05); middle dose can prolong remarkably the survival time of mice planted with H22 fluid tumor(P< 0.05); high dose, middle dose and low dose PePs could inhibit the tumor’s growth, the inhibition rate was up to 52.13%, 50.08%, 31.33% respectively. The middle dose and low dose PePs have no inhibit effect on immunologic function of mice.更多还原