【作者】 崔丽春；

【导师】 马泽芳； 李和平；

【作者基本信息】 东北林业大学 ， 野生动物保护与利用， 2004， 硕士

【摘要】 本试验采用高效液相色谱法(HPLC)对不同生理时期梅花鹿血液中抗衰老成分——尿嘧啶、次黄嘌呤、卵磷脂进行定性、定量研究。提供了尿嘧啶、次黄嘌呤、卵磷脂高效液相色谱分析条件。 试验结果表明： 1．梅花鹿不同生理时期尿嘧啶含量差异显著(P＜0.05)，说明梅花鹿不同生理时期对尿嘧啶的含量明显不同。其中，配种期、生茸期与生茸前期尿嘧啶的含量差异显著(P＜0.05)：配种期、生茸期与恢复期尿嘧啶的含量差异显著(P＜0.05)：其它各时期间尿嘧啶的含量差异不显著(P＞0.05)。 2．梅花鹿不同生理时期血液中次黄嘌呤含量差异不显著(P＞0.05)。说明梅花鹿血液中次黄嘌呤的含量与生理时期无关。 3．梅花鹿不同生理时期的血液中卵磷脂的含量差异极显著(P＜0.01)。说明梅花鹿不同生理时期血液中卵磷脂的含量有明显不同。其中，生茸前期与生茸期对配种期卵磷脂的含量差异极显著(P＜0.01)；恢复期对配种期卵磷脂的含量差异显著(P＜0.05)。 此研究将对鹿产品的合理开发与利用提供科学的理论依据，对推动我国养鹿业的发展具有重要意义。更多还原

【Abstract】 The experiment was designed to qualify and quantify the Uracil, Hypoxanthine,Phospholipid in the blood of sika deer in different physicological period and probe into the analytic methond of HPLC. The results of experiment are as following:1. The content of Uracil in three compositions had a mighty marked discrepancy(P<0.05) in the blood of Sika deer in different physiological period, which indicated the difference of physiological period of Sika deer had a remarkable impact on the content of Uracil. The diversity of the content of Uracil between the period of hybridization and the period of anter growing was mighty marked(P<0.05) in different physiological periods, the discrepancy of the content of Uracil between the period of hybridization and the period of renewing was marked(P<0.05); there was no marked discrepancy between other physiological periods(P >0.05).2. There was no discrepancy of the content of Hypoxanthine in different physiological periods, which shew the content of Hypoxanthine in the blood of Sika deer was irrelative to physiological period.3. The content of Phospholipid has a mighty marked discrepancy (PO.01) in the blood of Sika deer in different physiological period, which indicated the difference of physiological period of Sika deer had a mighty remarkable impact on the content of Phospholipid. The discrepancy of the content of Phospholipid between the pre-period of anter growing or the period of anter growing and the period of hybridization was mighty marked(P<0.01) in different physiological periods, the discrepancy of the content of Phospholipid between the period of hybridization and the period of renewing is marked(P<0.05).The study will provide a firm theorical foundation for the development of product of deer and promote the development of farming deer.更多还原