【作者】 景凤娟；

【导师】 黄楠；

【作者基本信息】 西南交通大学 ， 材料学， 2004， 硕士

【摘要】 采用微波电子回旋共振磁过滤弧设备制备钛氧薄膜。用双氧水与盐酸对Ti-O膜表面进行预处理后，在钛氧膜表面共价固定氨丙基三乙氧基硅烷(APTE)，然后通过APTE的活性氨基共价偶联白蛋白、肝素生物大分子。采用XPS、XRD、AFM、RAMAN分析钛氧膜表面成分及结构，采用XPS、FTIR分析钛氧膜表面APTE、白蛋白及肝素成分。对钛氧膜及其表面固定生物大分子后的表面做接触角测试及血小板粘附实验，用光镜与SEM观察血小板的数量与形态。另外在Ti-O膜表面直接固定吸附白蛋白与肝素，通过AFM，血小板粘附实验、纤维蛋白吸附实验、接触角测试来分析其表面特征。对钛氧膜及其表面固定生物大分子后的表面体外培养内皮细胞，以研究生物大分子膜对内皮细胞的作用与影响。结果表明，采用微波ECR磁过滤弧设备制备钛氧薄膜时，随着氧流量的增加，钛氧膜向金红石结构转变。退火后氧化钛晶粒增大，血液相容性得到了一定的提高。金红石型氧化钛薄膜具有与热解碳相当的血液相容性。Ti-O膜表面活化后，表面清洁并含有活性基团，而且血液相容性得到了改善。Ti-O膜可共价固定氨丙基三乙氧基硅烷(APTE)，从而使其表面含有活性氨基，并且厚度在纳米级以内。白蛋白与肝素可通过APTE共价固定于钛氧膜表面。白蛋白、肝素的共价固定可减少血小板粘附的数量与变形，从而改善其血液相容性。对钛氧膜表面直接吸附固定白蛋白，表明能够提高其血液相容性。钛氧膜表面固定白蛋白及肝素后，内皮细胞可在其表面粘附生长，但比起钛氧膜本身内皮细胞生长粘附能力并未增强。更多还原

【Abstract】 Ti-O film was prepared by microwave-electron convolute resonance (MW-ECR) magnetron filter arc deposition process. After the Ti-O film was pretreated by H2o2 and Hcl mixed solution, Aminopropyltriethoxysilane (APTE) was covalently interacted with hydroxyl on the surface of Ti-O film. Then albumin, heparin and albumin plus heparin were immobilized to the amidogen of APTE through covalent chemical attachment. X-ray photoelectxon spectroscopy (XPS), Atomic Force Microscopy (AFM), X-ray diffraction(XRD) and Raman spectroscopy (Raman) was used to investigate the characteristic of surface of Ti-O film. XPS and Fourier Transform Infrared spectroscopy (FTIR) was used to study the APTE, albumin and heparin on the surface of Ti-O film. The contact angle was measured and the behavior of blood platelet adhesion were studied by SEM after incubation of the samples in platelet rich plasma (PRP). In addition, the experiments of albumin directly immobilizing on the surface of Ti-O film were also performed. The seeding of endothelial cells on the surface of Ti-O film as well as on Ti-O films with albumin and heparin binding were investigated.The results revealed that with the increase of oxygen flows, the structures of Ti-O film tend to form rutile. After annealing the grain sides of the Ti-O film increased and blood compatibility improved. The rutile Ti-O film has the similar blood compatibility compared with the low temperature isotropic pyrolytic carbon. Active group of hydroxyl onTi-0 surface improved the blood compatibility. APTE with active amidogen could be covalently immobilized on the surface of Ti-0 film and its thickness is less than 100 nm. After covalently immobilizing of albumin and heparin on APTE coated Ti-0 film, behavior of blood platelet adhesion became better. Directly immobilization of Albumin on the surface of Ti-0 film also showed good compatibility. It was proved that endothelial cells could grow on the surface of Ti-0 film which immobilized with albumin and heparin. However the behavior of growth and proliferation of endothelial cell on biomolecular immobilized Ti-0 film was not as good as on naked Ti-0 film.更多还原