【作者】 牛一丁；

【导师】 哈斯阿古拉；

【作者基本信息】 内蒙古大学 ， 微生物学， 2004， 硕士

【摘要】 以拟南芥基因组DNA为模板，通过PCR扩增得到逆境胁迫诱导转录因子DREB1A基因，将其克隆到pUC19质粒中。序列分析表明获得的基因序列与已报道的该基因的序列完全相同。将DREB1A基因的5’端和拟南芥rd29A启动子片断相连，然后将该片断与pBI101.2的nos终止子片断共同构建到植物表达载体pPZP221中。 重组表达载体经农杆菌LBA4404介导转化烟草，获得具有庆大霉素抗性的转化体。经PCR检测，证明目的基因已整合到烟草基因组中。更多还原

【Abstract】 The stress-inducible transcription factor DREB1A gene is amplified from Arabidopsis thaliana (Columbia ecotype) genome by polymerase chain reaction and cloned into pUC19 vector. Sequence analysis showed that the obtained gene is identical to the reported sequence. Subsequencly, the rd29A promoter was fused to the upstream of the DREB1A. Then the fusion gene, rd29A-pro : DREB1A, and the nos terminator of the pBI101.2 vector were constructed into plant expressed vector pPZP221.Tobacco plants were transformed with the expression vector by Agrobacterium-medlated transformation. Then, the gentamycin-resistant tobacco transformants were obtained. PCR detection demonstrated that the fusion gene has been integrated into tobacco genome.更多还原