【作者】 袁海滨；

【导师】 史树森； 张永军；

【作者基本信息】 吉林农业大学 ， 农业昆虫与害虫防治， 2004， 硕士

【摘要】 2003年6月～2003年10月在中国农业科学院廊坊中试基地的实验田研究了转Bt基因棉花对龟纹瓢虫种群动态的影响，研究结果表明，转基因棉花田龟纹瓢虫的种群数量比非转基因棉花田龟纹瓢虫的种群数量高，在7月30日左右达到高峰，之后逐渐减少。 以转基因棉田蚜虫和非转基因棉田蚜虫为饲料，在室温下，单头饲养龟纹瓢虫，结果表明，个体发育历期无显著差异，只在个别虫态有差异。以转基因棉田蚜虫喂饲的龟纹瓢虫的存活率、成蛹率显著低于以非转基因棉田蚜虫喂饲的龟纹瓢虫。转基因棉田和非转基因棉田龟纹瓢虫2龄幼虫体重无显著差异，3龄、4龄幼虫及蛹的重量有显著差异。 比较转基因棉田和非转基因棉田龟纹瓢虫解毒酶和主要蛋白酶的活性。研究结果表明，除个别虫态转基因棉田龟纹瓢虫与非转基因棉田龟纹瓢虫个别解毒酶、蛋白酶活力无差异外，在棉花不同生长时期以及龟纹瓢虫不同虫态，转基因棉田龟纹瓢虫与非转基因棉田龟纹瓢虫α-乙酸萘酯酶、乙酰胆碱酯酶、谷光甘肽-S-转移酶、酸性磷酸酯酶、碱性磷酸酯酶的活性都存在显著差异。总蛋白酶、强碱性类胰蛋白酶、弱碱性类胰蛋白酶、类胰凝乳蛋白酶活的也都有显著差异。 用ELISA法测定龟纹瓢虫体内Bt毒蛋白的含量，在棉花的蕾期-花期，33B转基因棉田的龟纹瓢虫2龄、4龄幼虫体内可以检测到Bt毒蛋白，含量分别为4.89ng／g、13.36ng／g，其它龄期检测不到Bt毒蛋白。在棉花的花铃期-铃期，转基因棉田龟纹瓢虫1龄、2龄、3龄、4龄幼虫及蛹期均检测不到Bt毒蛋白。更多还原

【Abstract】 The population dynamics of Propylaea japonica (thunberg) on Bt cotton were preliminarily investigated from June to October, 2003 in Langfang City, Hebei Province of northern China. The results indicated that during growing stage of hosts, the population of thunbergs in Bt cotton field were much more than that in Non-Bt cotton field, which increased continually and up to a peak around on 30rd July, then gradually decreased.The results showed that there were no significant differences in the development duration among the thunbergs reared on Aphid which collected from Bt cotton field and Non-Bt cotton field, respectively. The survival rate of larvae and pupae that reared on Aphid which collected from Bt cotton field were significant lower than that reared on Aphid which collected from Non-Bt cotton field. There were significant differences in the body weight of third, fourth larvae and pupae, but no significant differences to the second larvae that reared on Aphid which collected from Bt cotton field and Non-Bt cotton field, respectively.The activities of detoxification enzymes and major protease isolated from Propylaea japonica were detected in the laboratory. The results indicated that except for several detoxification enzymes and major protease showed no differences on the activity between thunbergs collected from Bt cotton field and Non-Bt cotton field during several stage, there were significant differences on the activities of a -naphthylacetate esterases, acetylcholinesterases (AchE), glutathione-S-transferase, acid phosphatase(ACPH) and alkaline phosphatase(ALP) during the growing stage of cotton and thunberg. And there were significant differences on the activities of the total protease, active alkaline trypsin-like enzyme, weak alkaline trypsin-like enzyme and chymotrypsin-like enzyme between the thunbergs which collected from Bt cotton and Non-Bt cotton field, respectively.The content of Bt (Bacillus thurngiensis Berliner) insecticide protein (BtIP) in thunbergs was studied by ELISA in laboratouy. The results showed that there were obvious spatio-temporal changes of the contents of BtIP along the development process of Bt transgenic cotton. The BtIP could been detected in second and fourth larvae during the cotton squaring stage to flowering stage, and the contents were 4.89ng/g and 13.36ng/g, respectively. But the BtIP could not been detected in other stages of thunbergs. And during the cotton flower boll stage to boll stage, the BtIP also could not been detected in any stages ofthunbergs.更多还原