【作者】 张建东；

【导师】 王勋陵；

【作者基本信息】 西北大学 ， 植物学， 2004， 硕士

【摘要】 本文以大豆和玉米为实验材料，用CO₂激光辐照浸种后的湿种子，研究了CO₂激光辐照对植物的影响。研究结果表明： （1）不同功率密度CO₂激光（16mw／mm²、18mw／mm²、20mw／mm²）处理大豆种子，其中以18mw／mm²CO₂激光辐照效果为最佳。而相同功率密度（18mw／mm²）不同时间激光处理（45s、60s、75s、90s、105s）大豆种子，75s辐照效果最为明显。 （2）在以不同功率密度的CO₂激光辐照大豆种子后，经CO₂激光处理的大豆种子发芽率、幼苗株高、茎粗和百粒重等形态学指标均有提高。其中18mw／mm²CO₂激光辐照对大豆的效果最好。而相同功率密度（18mw／mm²）不同时间激光处理后，大豆的发芽率、株高、茎粗和百粒重等形态学指标也都有提高，时间为75s的处理对大豆的效果最佳，时间为105s的处理与对照相比，差异不显著。 （3）不同功率密度的CO₂激光辐照大豆种子，与对照相比，CO₂激光处理能够提高可溶性糖、可溶性蛋白和游离氨基酸的含量，使淀粉酶活性增强。18mw／mm²CO₂激光处理具有最佳的效果。而相同功率密度（18mw／mm²）不同时间激光处理后，上述指标也都有提高，相比之下，以75s处理的效果最好。高于或低于这一时间的处理，这些生化指标会出现下降，特别是当辐照时间过长（105s）部分指标与对照相比会出现下降，产生负面影响。 （4）不同功率密度的CO₂激光辐照和同功率密度（18mw／mm²）不同时间激光处理后大豆种子后。与对照相比，CO₂激光处理大豆的叶绿素含量增加，光和速率上升，气孔导度下降，水分利用率上升，光合能力提高。18mw／mm²CO₂激光处理具有最佳的效果。而相同功率密度（18mw／mm²）不同时间激光处理后，光和能力增强。以75s处理后，光和能力提高幅度最大。 （5）用功率密度为18mw／mm²的CO₂激光以不同时间辐照玉米的湿种子，选择30s、60s、90s三个时间段，以激光处理60s的效果最佳。 （6）功率密度为18mw／mm²的CO₂激光处理后玉米种子的发芽率、株高、茎粗和百粒重等形态学指标均有上升，且增幅随发育进程加大。其中以CO₂激光辐照60s的增幅最大。 （7）功率密度为18mw/ITunZ的co:激光处理后玉米种子，玉米幼苗中可溶性糖、可溶性蛋白和游离氨基酸的含量均有增加，淀粉酶和蛋白酶活性提高。与对照相比，305处理差异不显著，605和905处理差异显著。 （8）功率密度为18mwznunZ的co:激光处理后玉米种子，玉米中的抗氧化系统增强，抗氧化酶SOD、POD、CAT活性增强，Vc含量增加。提高了清除活性氧能力，使植物具有较高的抗氧化能力，能够进行正常的生理生化代谢。 （9）功率密度为18mwzmrnZ的co:激光处理后玉米种子，玉米叶绿素含量增加，光和速率上升，气孔导度下降，水分利用率上升，光合作用能力提高。 从以上实验结果可以看出，适当剂量的COZ激光处理，生理生化水平的提高可以使植物生长旺盛，光合作用能力增强，抗氧化系统活性增强。实验中初步得出了对大豆和玉米C02激光辐照较佳剂量，对生产实践有一定的应用价值。更多还原

【Abstract】 Using corn (Zea mays L.) and soybean (Glycine max Merr) as the experimental material in this paper, we use CO2 laser to radiate on the soybean and corn wet seeds and then observe the effects of seedlings development in the experiments. The findings are:(1) After the CO2 laser with different power density (16mw/mm2, 18mw/mm2, 20mw/mm2) radiates on the soybean seeds, compared with control, the treatment of 18mw/mm2 is the best one among three treatments. After the soybean was radiated with five different length of time (45s, 60s, 75s, 90s, 105s), the treatment of 75s is the best one among five treatments.(2) After different power density CO2 laser radiated on the soybeans, all treatments show that soybean morphological indexes such as germination capability, plant high, plant diameter, 100 seeds weight were increased. After different time of radiation on the soybeans, morphological indexes also were improved. The best was 75s.(3) After different power density CO2 laser radiated on the soybeans, compared with the control, the indexes such as soluble protein content, soluble saccharine content, amino acid content and amylase activity increased. The treatment of 18mw/mm2 is the best. After different time of radiation on the soybeans, above of indexes were also improved. We found that 75s was the best one among five treatments. When the time limit was longer or shorter than it, these indexes would come down. Especially when the radiation time was too long (105s), CO2 laser radiation would have negative effects on soybean.(4) After different power density CO2 laser radiated on the soybeans, chlorophyll content rose. At the same time, the photosynthetic rate (Pn) ascent and stomatal conductance descent of soybean have also promoted the water use efficiency (WUE). The treatment of 18mw/mm2 is the best. After different time of radiation on the soybeans, photosynthesis ability was improved, too. The treatment of 75s is the best one.(5) We chose 3 different time limits (30s, 60s, 90s). After different time of radiation (the treatment of 18mw/mm2) on wet corn seeds, compared with control, the treatment of 60s is the best one among three treatments.(6) The corn morphological indexes such as germination capability, plant high, plant diameter, 100 seeds weight were increased under different time CO2 laser treatment. With the development of corn seedlings, the increase extent grew bigger. The treatment of 60s is the best.(7) After different time of radiation on the corn seeds, the indexes such as soluble protein content, soluble saccharine content and amino acid content increased; Amylase and protease activities rose. The effects of the 30s’ treatment is small, others’ treatment effects are bigger than the control.(8) Under the treatment of different time radiation on the corn seeds, antioxidative enzymes SOD, POD and CAT activities rose; Vc content increased. These indexes increased the capable of scavenge of superoxide radical mechanism which made plants be able to grow normally.(9) After different time of radiation on the corn seeds, chlorophyll content rose. The photosynthetic rate (Pn) ascent and stomatal conductance descent made the water use efficiency higher.The conclusions of this paper are the primary results, which have some application values in product.更多还原