【作者】 王媛媛；

【导师】 李震；

【作者基本信息】 山东中医药大学 ， 中西医结合基础， 2004， 硕士

【摘要】 目的：探讨冬凌草醇提物对荷H₂₂瘤小鼠的抑瘤作用及其抑瘤机理。方法：采用体内实验法将H₂₂肝癌细胞悬液注入小鼠右腋皮下制成局灶性肿瘤模型，观察模型组、卡莫氟阳性对照组、冬凌草醇提物大、中、小剂量组荷瘤小鼠一般状况的改变；14天后比较各组小鼠瘤重并观察各组肿瘤组织细胞形态学变化；用免疫组化法检测各组凋亡相关基因bcl-2、bax、突变型p53的表达；采用原位杂交技术对各组端粒酶反转录酶活性的表达进行检测。结果：冬凌草醇提物大、中、小剂量组与模型组相比瘤体小，和卡莫氟阳性对照组作用相似；三个剂量组突变型p53基因、端粒酶反转录酶活性和bcl-2基因表达皆小于模型组，其中前两个指标三个剂量组间无显著性差异，后一指标中剂量组和小剂量组间存在显著性差异；三个剂量组bax基因表达皆高于模型组，且中剂量组和小剂量组间亦存在显著性差异。结论：冬凌草醇提物大、中、小剂量组对小鼠H₂₂肝癌有抑瘤作用。其机制可能与凋亡活化基因bax表达增高，凋亡抑制基因bcl-2以及突变型p53基因表达减少相关，同时可能与肿瘤细胞端粒酶反转录酶活性的表达减少有关。更多还原

【Abstract】 Objective: To explore the effect and mechanism of suppressing tumors with the alcoholic extract of Donglingcao. Methods: First we adopted the Kun ming pedigree mice inoculated with H22 liver cancer cells in axilla subcutaneous tissue as the transplanting model to study the effect of tumor-suppressing. Second the different doses of the alcoholic extract of Donglingcao were given to the mice orally. Finally we not only observed the changes of common status of the mice and morphologic changes of histocyte but also measured the gene expression of mutant p53, bcl-2 and bax with immunohistochemical method and the expression of activity of telomerase reverse transcriptase with hybidization in situ. Results: The results indicated that the effect of the treated groups was better than that of the control group. The study of its mechanism showed that the alcoholic extract of Donglingcao could increase the expression of bax gene and decrease the expression of bcl-2 gene, mutant p53 gene and the activity of telomerase reverse transcriptase. Conclusion: From the whole experiment, we draw our conclusion that the alcoholic extraction of Donglingcao can effectively suppress the growth of tumors. The mechanism might be related to the up-regulation of the bax gene expression and down-regulation of the bcl-2 gene ,mutant p53 gene and the activity of telomerase reverse transcriptase expression.更多还原