【作者】 高杉；

【导师】 陈志武； 赵维中；

【作者基本信息】 安徽医科大学 ， 药理学， 2003， 硕士

【摘要】 黄蜀葵总黄酮（total flavone of abelmoschus maniho L medic，TFA）是从锦葵科秋葵属植物黄蜀葵花中分离的有效部位。本文在多种不完全性脑缺血动物模型上观察了TFA的抗脑缺血作用，并探讨了TFA抗脑缺血损伤可能的机制。主要实验结果如下： 1、20、40、80 mg·kg^-1TFA ig给药，可明显延长小鼠断头后张口喘气时间，并呈一定的量效关系。 2、TFA25、50、100 mg·kg^-1 ig给药，能明显减轻大脑中动脉结扎（MCAO）致大鼠脑组织梗塞重量，100 mg·kg^-1 TFA可明显抑制脑梗塞组织LDH活性的降低。 3、电镜和TUNEL法检查结果表明，TFA 25、50、100 mg·kg^-1能明显抑制MCAO致大鼠脑细胞的凋亡，并呈一定的剂量依赖性。 4、TFA 25、50、100 mg·kg^-1能明显抑制MCAO致脑组织NO含量的降低，并呈一定的量效关系。 5、50、100 mg·kg^-1 TFA能明显减轻大鼠急性不完全性脑缺血后脑组织含水量，25、50、100mg·kg^-1 TFA能明显改善脑组织病理组织学改变。 6、TFA 15、30 mg·kg^-1能明显增加狗颈内动脉血流量，30 mg·kg^-1 TFA也能明显降低狗脑血管阻力，但15、30、60 mg·kg^-1 TFA对平均动脉压、心电图、心率无明显影响。 结论：TFA对不完全性脑缺血损伤有明显保护作用，其机制可能与抗凋亡，提高脑组织中NO含量，扩张血管增加脑血流量有关。更多还原

【Abstract】 Total flavones in the flowers of abelmoschus manihot L (TFA) is the effective part, which was extracted from a Chinese herb called abelmoschus manihot L. In this paper, the protective effect of TFA on incomplete cerebral ischemia injury and its mechanism were studied.Results were showed as followed:1. 20, 40, 80 mg. kg-1 TFA can significantly and dose-dependtently prolong the time of gasping after cutting head in mice.2. On MCAO (middle cerebral artery occlusion) model, TFA (25,50,100 mg-kg-1,ig) can significantly reduce the weight of cerebral infarction . TFA 100 mg-kg-1 can also inhibit the decrease of the activity of LDH in the cerebrum.3. On MCAO model , the examination of transmission electron microscopy and TUNEL method showed that TFA (25,50,100 mg-kg-1,ig) can dose dependently inhibited the apoptosis of brain cells4. On MCAO model, TFA (25,50,100 mg-kg-1,ig) remarkably increased NO content in the cerebrum in dose-dependent manner.5. On the acute incomplete cerebral ischemia model in rats, TFA (50,100 mg-kg-1,ig) can obviously reduce the water in the cerebrum, and 25,50,100 mg-kg-1 improve the pathology changes of cerebral tissue.6. TFA 15,30 mg kg-1 can increase the cerebral blood flow in dogs, and 30 mg ’kg-1 TFA significantly reduced the peripheral resistance of cerebral vessels, on the other hand ,15,30,60 mg kg-1 TFA has no obvious effects on mean arterialpressure, electrocardiogram (ECG) and heart rate in dogs. As above, TFA play a protective role on incomplete cerebral ischemia injury via inhibition of apoptosis, increase the cerebral NO content , relaxing of cerebral vessel and increasing cerebral blood flow.更多还原