【作者】 陈伟；

【导师】 许建明；

【作者基本信息】 安徽医科大学 ， 内科学， 2003， 硕士

【摘要】 目的 通过研究早期、中期、晚期胃癌和不典型增生及慢性胃炎中NF-κBp65、VEGF表达，探讨胃癌发生、浸润和转移过程中NF-κBp65、VEGF的表达和MVD的关系及其临床意义。方法 收集马鞍山市马钢医院和六安市人民医院1997年-2002年经术后病理证实的早期、中期和晚期胃癌62例作为研究对象，同时将35例不典型增生及慢性胃炎作为对照组，进行免疫组织化学染色。检测其中NF-κBp65、VEGF的表达和MVD，结合随访资料，分析它们之间的关系及其临床意义。结果 胃癌组中NF-κBp65、VEGF的阳性表达率分别为62.9％、51.6％，而对照组的NF-κBp65、VEGF的阳性表达率仅为17.1％、11.4％，胃癌组与对照组NF-κBp65、VEGF的阳性表达率差异有显著性(P＜0.01)。同时胃癌组中的MVD明显增高(P＜0.01)；胃癌组中，早期、中期和晚期胃癌之间NF-κBp65、VEGF表达阳性率和MVD差异有显著性(P＜0.05)。NF-κBp65表达与VEGF表达呈正相关(γs=0.593，P＜0.001)。胃癌中有淋巴结转移组VEGF的表达和MVD明显高于无淋巴结转移组(P＜0.05)。结论 胃癌中NF-κBp65、VEGF阳性表达和MVD增高，与肿瘤的浸润程度，淋巴结转移和患者预后密切相关，是影响胃癌患者预后的重要因素。NF-κBp65、VEGF表达与MVD有显著相关，提示NF-κBp65、VEGF对肿瘤血管生成有重要作用，与肿瘤的发生、浸润和转移有密切关系。更多还原

【Abstract】 Objection To investigate the expression of NF- Bp65,VEGF and MVD in humor gastric carcinoma and control group (chronic gastritis and gastric mucosal atypical hyperplasia), to study relationship and clinic significance between expression of NF- Bp65 , VEGF and MVD in human gastric carcinoma . Methods 62 patients with gastric carcinoma who underwent surgical resection between 1997 and 2002 at the Ma Gang hospital and the hospital in Liu An were followed up . Expression of NF-icBp65,VEGF and MVD were examined by S-P immunohistochemistry in 62 archival paraffin embedded gastric carcinoma tissue specimens and 35 patients of control groups. The relationship between expression of NF- Bp65, VEGF and MVD in human gastric carcinoma and control groups were analyzed by SPSS 10.0 version software .Results The expression of NF- Bp65, VEGF were obviously higher in gastric carcinoma groups than in the control group (62.9% versus 17.1%, 51.6% versus 11. 4%, respectively, P<0. 001) . The difference between expression of NF- Bp65 , VEGF and MVD in different stages of gastric carcinoma were significant (P<0. 05) . The expression of NF-icBp65 was significantly correlated with VEGF ( Y s=0. 593, P<0. 001) . The expression of VEGF and MVD were significantly higher in lymph-node- metastases group than in no-lymph-node metastases group (P<0. 05) . Theexpression of NF-KB p65 and VEGF were highly correlated with MVD ,which was significantly higher in the NF-icB p65(+) group and VEGF (+) group (P<0. 001) . Conclusion The expression of NF-icBp65 ,VEGF and MVD were associated with the different stages of gastric carcinoma, lymph-node metastases and prognosis, NF-KBp65. VEGF and MVD may serve as the important prognostic factors in gastric carcinoma. Expression of NF-icBp65and VEGF were highly correlated with MVD .The relationship between NF-icBp65, VEGF and MVD indicates that NF-icBp65, VEGF may play a important role in occurrence and metastases of gastric carcinoma更多还原