【作者】 严萍；

【导师】 周河治；

【作者基本信息】 广西大学 ， 发酵工程， 2004， 硕士

【摘要】 本人自行筛选分离得到一株菌株Y32，能产生活性较强的纤溶酶，有望发展为新型的栓溶剂。本研究以Y32为出发菌，通过常规育种方法对其孢子悬液进行了一系列的诱变，经过大量筛选，选育出一株高产菌株Yc1；并探讨其发酵条件及酶学性质。 从南方的一种食品中筛选的得菌株Y32，初步鉴定为根霉。首先对该菌株进行紫外线诱变，得菌株Y13，然后对Y13进行Co⁶⁰诱变，得菌株Yc1，其酶活力为53.845尿激酶IU／ml，比原种提高了63.8％。 运用正交实验设计法对培养基的组分进行综合考虑，较佳的发酵培养基配方为（％）：麸皮5.0，黄豆粉3.0，ZnCl₂0.03，MgSO₄·7H₂O0.15，Na₂HPO₄·12H₂O 0.05，K₂HPO₄·3H₂O 0.6，pH为7.0，40ml／250ml装液量，12小时种子培养液，接种量为10—15％，30℃，160rpm发酵培养60h，酶活达163.059尿激酶IU／ml，比原发酵条件提高202.83％。 运用硫酸铵分级盐析，从发酵液中分离出主要的纤溶酶。更多还原

【Abstract】 Yd was screened in nature, which can produce a fibrinolytic enzyme. The fibrinolytic enzyme will be developed as a thrombotic agent. A series of treatment has been done to the initial strain Y32, to bread a new mutant梇c1. It’s condition of characterization and fermentation was investigated.Y32 was identified as Rhizopus sp. by its morphology. The fibrinolytic enzyme activity of Yc1 reached 53.845UK/ml, increasing 63.8 fold.By the method of orthogonal test, the optimal main medium was achieved, which contained 5.0% wheat bran, 3.0 % soybean meal, 0.03% ZnCl2.0.15% MgS04.7H20, 0.05% Na2HP04.12H20, 0.6 %K2HP04.3H20, pH 7.0. The Yc1 was inoculated into 40ml medium in 250ml triangle flask shaking in 30℃ at 160rpm for 60h. The fibrinolytic enzyme activity of Yc1 reached 163.059UK/ml increased 202.83 fold.A fibrinolytic enzyme was purified from the fermented broth of Yd by ammonium sulfate fractional precipitation.更多还原