【作者】 白坤栋；

【导师】 许鸿源；

【作者基本信息】 广西大学 ， 作物栽培学与耕作学， 2004， 硕士

【摘要】 为建立木薯的化学调控技术，并为其提供理论根据，本研究在分别筛选出吲哚丁酸（IBA）和氯化胆碱（CC）最适宜使用浓度的基础上，设计了3组对比试验：①50mg·L^-1IBA浸种；②块根膨大初期喷施300 mg·L^-1CC；③50mg·L^-1IBA浸种+块根膨大初期喷施300mg·L^-1CC，以H₂O作CK。结果表明： （1）IBA和CC单独使用及配合使用都能促进木薯光合作用，其促进程度顺序为：IBA+CC＞CC＞IBA＞CK。 IBA浸种与CK相比，Chla、Chl（a+b）和Car含量有所提高，Chlb含量显著提高，Chla／Chlb和Chl／Car比值得到改善；Fo、Fm、Fv／Fm和Fv／Fo略有增加；对Tr、Gs、Ci和Pn有显著的促进效应。 喷施CC与CK相比，Chla、Chlb和Chl（a+b）含量显著提高、Car含量增幅不显著，Chla／Chlb和Chl／Car比值得到进一步改善；Fo、Fm、Fv／Fm和Fv／Fo增幅有限，但显著促进Tr、Gs、Ci和Pn。 IBA和CC配合使用与CK相比，大幅度地显著增加Chla、Chlb和Chl（a+b）含量，对Car的促进效应依然不显著，但却使Chla／Chlb和Chl／Car比值更能适应高光合作用的要求；Fo、Fm、Fv／Fm和Fv／Fo进一步提高，而且对Tr、Gs、Ci和Pn皆达到极显著的促进作用。 广1盯大学硕士论文.5I嗓勺r胶和氮化胆碱对木落化学润挽机理的研究 （2）IBA和CC单独使用及配合使用均可提高木薯营养水平，其 增幅顺序为:旧A+CC>CC>!BA>CK。 IBA和CK相比，叶片总氮、可溶性蛋白质和可溶性糖含量有所;提高，但未达到显著水平。喷施cc与cK相比，叶片总氮、可溶性蛋 白质和可溶性糖含量都显著提高。旧A和cc配合使用与cK相比，更 是极显著的提高了叶片总氮、可溶性蛋白质和可溶性糖含量。 木薯营养水平的提高，与其促进光合效率、增加青叶数、扩大受 光面积、推迟叶片衰老及延长光合时间，从而增加同化物的累积是一 致的。 （3）IBA和CC单独使用及配合使用明显提高木薯抗逆潜力，其 提高的顺序为:IBA+CC)CC>旧A>CK。 IBA浸种和CK相比，显著抑制叶片细胞电解质的相对外渗率， 显著提高P0活性;MDA含量有所降低，SOD、poo和CAT等抗氧化酶 的活性提高，但都没有达到显著水平。 啧施cc与cK相比，显著降低叶片细胞电解质的相对外渗率和 MDA含量，同时显著提高SOD、POD、CAT和PO等抗氧化酶的活性。，IBA和CC配合使用与CK相比，叶片细胞电解质的相对外渗率和 1一’MoA含量显著降低，500和e盯活性显著增加，poo和po活性极显 著的提高。 同时，Car、Pr。等非酶性自由基清除剂的含量及可溶性糖、可 溶性蛋白质等渗透调节物质的浓度也得到提高，对木薯抗逆潜力的提 高也有重要作用。广卫『大学硕d卜论文，于嗓勺，胶和每化胆碱月宁洲仪落化学润挽机理的花玲究 上述结果证明，旧A与cc配合使用比其分别单独使用能够更好 地改善木薯植株的内在生理状态，而且最终取得高产优质的效果。 提高木薯块根产量的顺序为:旧A+CC>旧A>CC>cK。单独喷施cc， 小区产量比对照显著提高14.6%;18A浸种，小区产量比对照显著提 高21.9%;旧A和CC配合使用，小区产量比对照极显著提高33.8%。 木薯块根淀粉含量的顺序为:旧A+cC>CC>CK>旧A，但各处理之间 的差异不显著。!BA浸种，块根淀粉含量低于对照1 .4%;单独用cc 喷施，块根淀粉含量高于对照1 .7%;旧A和CC配合使用，块根淀粉 含量比对照提高7.1%。 提高木薯折每亩淀粉产量的顺序为:旧A+cc>旧A>cc>cK。单独用 啧施cc，折每亩淀粉产量显著地高干对照1 6.6%;!BA浸种，折每 亩淀粉产量显著的高于对照20.2%;旧A和CC配合使用，折每亩淀‘ 粉产量则极显著地比对照提高43.4%、比喷施cc和IBA浸种分别显 著提高26.3%和19.4%。更多还原

【Abstract】 In order to establish the chemical regulation technology of cassava and provide its theoretical basis, this study designed three treatments of contrastive experiments on the base of having selected the most appropriate concentrations of IBA and choline chloride(CC) : (1) soaking the cuttings of seed stems w i th 50 mg L-1 IBA ; (2), spray i ng 300 mg L-1 CC on the leaves at the first expanding stage of tuber growth ; (3), soaking the cuttings of seed stems with 50 mg L-1 IBA + spraying 300 mg L-1 CC on the leaves at the first expanding stage of tuber growth, taking H2O as control. The research findings were presented briefly as follows.(1)The single and cooperative applications of IBA and CC all accelerated the photosynthesis of cassava, the sequence of such applications was IBA+CC>CC>IBA>CK.Compared with control, soaking the cuttings of seed stems with IBA increased the contents of Chla, Chl (a+b) and Car tosome extent, increased the Chlb content significantly and improved the values of Chla/Chlb and Chl /Car. Fo, Fm, Fv/Fm and Fv/Fo were all enhanced siightly while Tr, Gs, Ci and Pn were all accelerated significantly.Compared with control, spraying CC increased the contents of Chla, Chlb and Chl (a+b) significantly, increased the Car content alittle and so further improved the values of Chla/Chlb and Chl/Car. Fo, Fm, Fv/Fm and Fv/Fo were al enhanced to finite extent whi Ie Tr, Gs, Ci and Pn were all promoted significant Iy.Compared with control, the cooperative applicat ion of IBA and CC increased the contents of Chla, Chlb and Chl (a+b) significantly to a further extent, still increased the Car content siightly,but made the values of Chla/Chlb and Chl/Car more adapatable to high photosynthesis. Fo, Fm, Fv/Fm and Fv/Fo were all added more whi le Tr, Gs, Ci and Pn were all promoted most significantly.(2) The single and cooperative applications of IBA and CC all could increase the nutrient levels of cassava, the sequence of such applications was IBA+CC>CC>IBA>CK.Compared with control, soaking the cuttings of seed stems with IBA increased the total nitrogen, the soluble proteins and the soluble sugars in the leaves to a less significantextent. Spraying CC increased the total nitrogen, the soluble proteins and the soluble sugars in the leaves significantly. The cooperative application of IBA and CC could improved the total nitrogen, the soluble proteins and the soluble sugars in the leaves most significantly.The i ncrease of nutrient levels of cassava so obtained was related to the strengthening of photosynthesis, the increase in the number of green leaves and light-accepted leaf area and the prolonging time of photosynthesis.(3 ) The single and cooper ative applications of IBA and CC all could strengthen the stess-resistant potential.The sequence of such applications was IBA+CC>CC>IBA>CK.Compared with control, soaking the cuttings of seed stems with could restrain the relative leakage rate of electrolytes significantly, i ncrease the PO activity significantly, decrease the MDA content and advance the activities of anti-oxidized enzymes such as SOD, POD and CAT.Compared with control, spray ing CC could reduce the relative leakage rate of electrolytes and the MDA content significantly. At the same time, the activities of anti-oxidized enzymes such as SOD, POD, CAT and PO were improved significantly.Compared with control, the cooperative appiicat ion of IBA and CC could decrease the relative leakage rate of electrolytes and the MDA content significantly, increase the activities of SOD and CAT significantly and heighten the activities of POD and PO to most significantly extent.Meanwhile, the growing contents of non enzyme-catalyzed free radical removalists such as Car and Pro and the climbing concentrations of osmotic adjustment substances such as soluble sugars and proteins were also responsible for the strengthening stress-resistant potential of cassava.Such results proved that the cooperative application of IBA and CC had more effect than the single applications of IBA and CC o更多还原