【作者】 张晓燕；

【导师】 王和平；

【作者基本信息】 黑龙江中医药大学 ， 药剂学， 2004， 硕士

【摘要】 酸枣仁为鼠李科植物酸枣Ziziphus jujuba Mill．var．spinosa(Bunge)Hu ex H．F．Chou的干燥成熟种子。具有补肝，宁心，敛汗，生津等功效，用于虚烦不眠，惊悸多梦，体虚多汗，津伤口渴。酸枣仁皂苷A、B是中药酸枣仁中的主要有效成分，并具有多方面的药理作用。 本文采用比较实验法确定了提取酸枣仁皂苷的最佳工艺为：甲醇回流6h，水饱和正丁醇萃取15ml／次，正丁醇饱和氨试液洗涤。并通过柱层析方法分离酸枣仁皂苷A、B，得到的粗品为Ⅰ、Ⅱ、Ⅲ、Ⅳ，为进一步精制打下了基础。 本课题主要通过HPLC-ELSD法测定了酸枣仁皂苷的含量，并采用正交实验设计确定了炒制酸枣仁的最佳条件，以确定其使用价值。通过对生、炒酸枣仁皂苷的含量测定表明二者的皂苷含量无显著差异。 此外对生、炒酸枣仁药材进行了系统的质量标准研究，测定了灰分、水分、浸出物、酸败度等各项指标。同时，对皂苷A、B进行了定性薄层鉴别。并以酸枣仁皂苷A、B含量为主要指标，追踪其炮制品在0，1，2，3，6，12个月的稳定性。结果表明：炒酸枣仁在一年内稳定，无虫蛀霉败现象，从而确定统一的标准，使酸枣仁及其炮制品达到准入的要求和产业化目标。更多还原

【Abstract】 Seed of wild jujube is the dry mature seed of Ziziphus jujuba Mill.var.spenosa (Bunge) Hu ex H.f.Chou. It can nourish hepar, arrest sweating, promote fluid production and induce sedation. Jujuboside A , B, the most effective components of seed of wild jujube, have many pharmacological effects.The best extract process of Jujuboside was determined by comparing several methods. The seed of wild jujube was refluxed with methanol for 6 hours, abstracted with macroporous resin once 15ml and washed out with Ammonia solution. Then, the extracts I , II, III, IV were separated with silica gel column chromatography.To confirm the worth of seed of wild jujube, quantity of Jujuboside was analysis by HPLC-ELSD. Then the process of the stir-baked seed of wild jujube was optimized by orthogonal design. The result showed that the quantity of Jujubosides in raw seed had no significant difference from that in the stir-baked one.To study the quality of seed of Jujube and its preparation completely, the quantity of inorganic matter in plants , water content and extractives were analyzed. Jujuboside A, B were identified by TLC. The quantity of Jujuboside A and B were assayed respectively in 0, 1, 2, 3 , 6 , 12 months. The results indicated that without moth-eaten and mycotic infection, the stir-baked seed of wild jujube had stable component quantity in one year. This study made seed of wild jujube and its processed products have confirmed standard to be used in market and industrial production.更多还原