【作者】 太成梅；

【导师】 匡海学；

【作者基本信息】 黑龙江中医药大学 ， 中药学， 2004， 硕士

【摘要】 北洋金花为茄科曼陀罗属植物毛曼陀罗Datura innoxia Mill.的干燥花。临床上常用其作为《中国药典》收载的正品洋金花[白曼陀罗（Datura metel L.）的花]的代用品应用。为了阐明两者化学成分的异同，本文对北洋金花进行了化学成分研究。 本文采用阳离子交换树脂、大孔吸附树脂柱色谱等方法精制、富集北洋金花的非生物碱水溶性组分，并应用硅胶柱色谱、聚酰胺柱色谱、ODS反相柱色谱等方法进一步进行分离。结果共分离得到8个单体化合物。结合理化分析和通过对其ESI-MS谱、¹H-NMR谱、¹³C-NMR谱、DEPT谱的综合解析，鉴定其中6个单体化合物化学结构分别为7-O-α-L-鼠李吡喃糖基-山柰酚（7-O-α-L-ramnopyranosyl-Kaempferol）（Ⅰ）；7-O-β-D-葡萄吡喃糖基-山柰酚（7-O-β-D-glucopyranosyl-Kaempferol）（Ⅱ）；3-O-β-D-葡萄吡喃糖基、7-O-α-L-鼠李吡喃糖基-山柰酚（3-O-β-D-glucopyr anosyl，7-O-α-L-ramnopyranosyl-Kaempfer-ol）（Ⅲ）；3-O-β-D-葡萄吡喃糖基（1→2）-β-D-葡萄吡喃糖基-山柰酚[3-O-β-D-gluco pyranosyl-（1→2）glucopyranosyl-Kaempferol]（Ⅳ）；3-O-β-D-葡萄吡喃糖基（1→2）-β-D-葡萄吡喃糖基、7-O-α-L-鼠李吡喃糖基-山柰酚[3-O-β-D-glucopyranosyl（1→2）-glucopyranosyl，7-O-α-L-ramnopyranosyl-Kaempferol]（Ⅴ）和3-O-β-D-葡萄吡喃糖基（1→2）-β-D-葡萄吡喃糖基、7-O-β-D-葡萄吡喃糖基-山柰酚[3-O-β-D-glucopyranosyl（1→2）glucopyranosyl，7-O-β-D-glucop yranosyl-Kaempferol]（Ⅵ）。这6个化合物均为首次从北洋金花中分离获得，同时证明，北洋金花的黄酮类的种类与洋金花基本相同。 本文确定结果可为阐明北洋金花和洋金花化学成分异同，进而深入比较两者功效的异同，以及开发利用北洋金花的资源提供了有价值的科学依据。更多还原

【Abstract】 Datura innoxia is dry flower of Datura innoxia Mill. It belongs to the family of Solanaceae. And it can be the substitute of Datura metel L.which is recorded in the <Chinese Pharmacopoeia>.To illustrate the difference of their Chemical constituents the article studied ones of Dutura innoxia Mill..By ion exchange resin 732(001x7) and AB-8 Macropore Adsorb ing resin the water-solubility component except alkaloid was refined and enriched.The further separation was made by using silicagel column chromatography, ODS column chromatography and polyamide chromatography.As a result,eight compounds (1-8) had been isolated from the 50% effective fraction of treatment psoriasis, using 1H-NMR,13C-NMR,compounds:7-O-a-L-rhamnopyranosyl-Ka empferol,7-O-β-D-glucopyranosyl-Kaempferol,3-O-β-D-glucopyran osyl,7-O-a-L-rh-amnopyranosyl-Kaempferol,3-O-β-D-glucopyranos yl-(1-2)-β-D-glu-copyranosyl-Kaempferol,3-O-β-D-glucopyranos-yl-(1-2)-β-D-glucopyranosyl,7-O-a-L-rhamnopyranosyl-Kaempfer ol and 3-O-β-D-glucop yranosyl (1-2)-p-D-glucopyranosyl,7-O-B -D-glucopyranosyl-Kaempferol.The compounds (1-6) were isolated from Datura innoxia Mill, for the first time.At thesame time,the sort of flavonoid in Datura innoxia Mill.and Datura metel L.is essentiall y the same.The result from this article can offer valuable scientific basis f or illuminating the similarities and differences between the chemica 1 constituants of Datura innoxia and Datura metel L., comparing thei r efficiency , and empoldering the resources of Datura innoxia.更多还原