【作者】 叶红；

【导师】 陈树德；

【作者基本信息】 华东师范大学 ， 生物物理， 2004， 硕士

【摘要】 本文以中国红豆杉细胞为植物细胞模型，研究了脉冲电场对细胞生长和次生代谢物积累的影响。不同生长时相的红豆杉细胞在不同时间的脉冲电场（50Hz，10V／m）作用下，其生长和胞内一种有生物活性的次生代谢物——云南紫杉烷（Tc）的积累表现出明显差异。对数前期的细胞经30min的电场诱导后，细胞生长没有明显变化但紫杉烷的含量（一种基于细胞干重的产量表达方式）提高了近30％。同时研究还发现，脉冲电场和补糖的组合策略能更有效地促进细胞积累紫杉烷，电场和生物诱导剂甲基茉莉酮酸酯（MJA）存在一定的协同作用。脉冲电场作用后活性氧（包括O₂^-和H₂O₂）的大量增加表明脉冲电场诱发了植物的防御反应，进而引起了胞内紫杉烷含量的提高。脉冲电场作为一个外部刺激或应力，将有希望成为一种新型的非生物诱导剂有效地提高植物细胞培养中次生代谢物的积累。更多还原

【Abstract】 Effects of pulsed electric field (PEF) on the growth and secondary metabolite production by plant cell culture were investigated by taking suspension cultures of Taxus chinensis as a model system. The cultured cells in different growth phases were exposed to a PEF (50Hz, 10V/m) for various periods of time. A significant increase hi intracellular accumulation of taxuyunnanine C (Tc), a bioactive secondary metabolite, was observed by exposing the cells in early exponential growth phase to a 30-min PEF. The Tc content (i.e. the specific production based on dry cell weight) was increased by 30% after exposure to PEF, without loss of biomass compared with that of the control. Combination of PEF treatment with sucrose feeding was proven useful in improving the secondary metabolite formation. And PEF had some synergistic effect with biotic elicitor-MJA. Production of reactive oxygen species was increased with PEF treatment. The results show that PEF induced the defense response of plant cells. PEF, an external stimulus or stress, is proposed as a promising new abiotic elicitor for stimulating secondary metabolite biosynthesis in plant cell cultures.更多还原