【作者】 伍楚蓉；

【导师】 韩萍；

【作者基本信息】 广西医科大学 ， 消化内科， 2004， 硕士

【摘要】 目的：探讨P53、P63、P73蛋白表达在胃癌发生发展中的可能机制。方法：选择57例手术切除的胃癌及胃癌旁组织蜡块标本，采用免疫组化方法检测组织中的P53、P63、P73蛋白表达，同时用Tunel方法检测胃癌组织中的凋亡细胞。结果：P53蛋白在胃癌中的表达率为38.6％，癌旁组织的表达率为17.5％(x~2=14.3242，P＜0.05)；P63蛋白在胃癌中的表达率为50.9％，癌旁组织的表达率为8.8％(x~2=34.6768，P＜0.05)；P73蛋白在胃癌中的表达率为36.8％，癌旁组织的表达率为5.3％(x~2=24.4598，P＜0.05)。胃癌组织中凋亡细胞为AI=5.7±4.3，癌旁组织凋亡细胞(AI=11.5±4.1)明显多于胃癌组织(P＜0.05)。胃癌组织P53、P63、P73蛋白表达率显著高于胃癌旁组织(P＜0.05)；低、未分化型腺癌表达率显著高于高、中分化型腺癌(P＜0.05)；有淋巴转移组显著高于无转移组(P＜0.05)；晚期胃癌P53、P63、P73的表达显著高于早期胃癌(P＜0.05)；胃癌组织P53、P63、P73蛋白表达三者间无相关(P＞0.05)。 结论：胃癌组织P53、P63、P73蛋白表达水平增高，可促使细胞凋亡受阻抑；细胞凋亡机制障碍可能是胃癌细胞增生失控的基础。更多还原

【Abstract】 Objective: The effect of gene P53, P63, P73 protein expression on the cellular apoptosis was studied for understanding the possible mechanism of malignant growth of gastric cancer.Methods:57 paraffin-embeded samples of gastric cancer and the para-cancer tissue from surgical resected tissues were selected. The expression of P53.P63 and P73 protein were detected immunohistochemically,and the Apoptosis in these samples was detected by Tunel labeling method. Results: Positive rate of P53 protein in gastric cancer samples was 38.6%. Positive rate of P63 protein in gastric cancer sample was 50.9%. Positive rate of P73 protein in gastric cancer sample was 36.8%.Those of P53,P63 and P73 in matched paracancer tissues were 17.5%,8.8% and 5.3% respectively.The AI(apotopsis index) in the paracancer tissues was significantly higher than those in the cancer tissues.The expression levels of P53.P63 and P73 correlated statistically with the histological grade and lymph node matastasis.and so did the AI.Furthermore,there was no significant relationship between the P53,P63 and P73 protein expression . Conclusions: The higher expression of P53,P63 and P73 in the gastric cancer may be contributed to the inhibition of cell apoptosis.and the abnormal mechanism of apoptosis may be lead to the proliferation of gastric cancer be out of control.更多还原