Survivin在人乳腺癌中的表达及As₂O₃的抑制作用

【作者】 马银斌；

【导师】 吴诚义；

【作者基本信息】 重庆医科大学 ， 外科学， 2004， 硕士

【摘要】 目的：抗凋亡基因Survivin于1997年首次报道，国内外对其在人乳腺癌中的表达了解甚少且存在争议，我们进一步探导Survivin在人乳腺浸润性导管癌中的表达及其临床意义。方法：收集病理标本，未经化学治疗的人乳腺浸润性导管癌28例; 经化学治疗的人乳腺浸润性导管癌17例；另选癌旁正常组织15例、乳腺纤维囊性增生10例、乳腺纤维腺瘤10例作为对照。用原位杂交、免疫组化检测上述组织中Survivin mRNA及Survivin蛋白的表达。结果：Survivin的原位杂交、免疫组化法检测结果无显著性差异且呈显著正相关；未经化学治疗的人乳腺浸润性导管癌病例中，Survivin mRNA、Survivin 蛋白总的表达均高，分别为（78.54±6.64）%、（76.69±5.78）%，不同年龄、TNM分期、病理分级、淋巴结转移、受体类型组间有显著性差异（p<0.001）；化疗后Survivin mRNA、Survivin 蛋白总的表达降低，分别为(64.16±5.47)%、(61.23±4.59)%，与未化疗组比较，有显著性差异（p<0.001）；而Survivin mRNA，Survivin蛋白在癌旁正常组织中无表达,在乳腺良性病变中显著低表达。结论：Survivin在人乳腺浸润性导管癌高表达，有利于其诊断及疗效的判定。更多还原

【Abstract】 Objective: Anti-apoptosis gene Survivin was first reorted in 1997,but still little has been known about the expression of Survivin in human breast cancer. The expression and significance of survivin in human breast infiltrating duct carcinoma was investigated in our studies.Methods: Tweenty-eight samples of human breast infiltrating duct carcinoma without chemotherapy, seventeen human breast infiltrating duct carcinoma with chemotherapy, Fifteen human normal breast tissue adjacent to carcinoma, Ten breast fibrous cystic hyperplasia and Ten breast fibroadenoma were collected; and the expressions of Survivin mRNA and Survivin protein in above-mentioned samples were determined by methods of in situ hybridization and immunohistochemistry. Results: The result of in situ hybridization for Survivin was positively correlated with that of immunohistochemistry without significant difference between the results of two methods.The positive rate of total expressions of Survivin mRNA and Survivin protein in human breast infiltrating duct carcinoma without chemotherapy was impressively high<WP=8>（78.54±6.64%、76.69±5.78%）and they were significantly different among the ages,TNM stages, pathology grades, lymph node metastases and types of receptor（p<0.001）；the totale expressions of Survivin mRNA and Survivin protein were decreased in human breast infiltrating duct carcinoma after chemotherapy（64.16±5.47%、61.23±4.59%）， and appeared significantly different from those of carcinomas without chemotherapy（p<0.001）; however, the expressions of Survivin mRNA and Survivin protein were not found in normal breast tissue and were very low in breast fibrous cystic hyperplasia and breast fibroadenoma.Conclusion: The expressions of Survivin is rather high in human breast infiltrating duct carcinoma and may be advantageous in diagnois of the disease, determination of therapeutic effect.更多还原