【作者】 张波；

【导师】 吴小翎；

【作者基本信息】 重庆医科大学 ， 内科学， 2004， 硕士

【摘要】 目的：探讨VEGF、CXCR4和SDF-1在胃癌组织中的表达及其在胃癌发生发展中的作用；通过分析它们之间的关系，初步探讨VEGF、CXCR4和SDF-1可能的作用机制。方法：应用免疫组化S-P法检测128例不同胃粘膜病变组织中的VEGF、CXCR4及 SDF-1蛋白表达。结果：胃癌组织VEGF和CXCR4的表达阳性率分别为64.29%和50.00%，显著高于正常胃粘膜、慢性胃炎、肠上皮化生和不典型增生组织（P＜0.05）；胃癌组织中SDF-1的表达阳性率为48.21%，显著低于正常胃粘膜、慢性胃炎和肠上皮化生（P＜0.05），与不典型增生组织相比，差异无显著性（P＞0.05）。胃癌组织VEGF和CXCR4的表达与患者的性别、年龄及肿瘤的大小和分化程度无显著相关性（P＞0.05），与浸润深度、淋巴结转移及临床分期有显著相关性（P＜0.05）；胃癌组织SDF-1的表达与临床病理特征之间未见显著相关性（P＞0.05）。胃癌VEGF表达与CXCR4表达呈正相关（γ＝0.452，P＜0.01），但两者与SDF-1表达未见相关性（P＞0.05）。结论：胃癌VEGF和CXCR4表达增高， VEGF除了促进肿瘤新<WP=7>生血管形成以外，还可能通过自分泌信号途径和CXCR4表达促进胃癌的浸润和转移；胃癌中SDF-1表达降低，可能是胃癌逃避机体免疫监视或免疫应答的一种机制，与胃癌的发生有关。更多还原

【Abstract】 Objective: To investigate the expression and significance of VEGF, CXCR4 and SDF-1 in gastric cancer.Method: The expression of VEGF,CXCR4 and SDF-1 in gastric cancer and precancerous leisions were examined with immunohisto- chemical techniques (S-P).Result: The positive rate of VEGF and CXCR4 in gastric cancer were 64.29% and 50.00%,and expressions of both were higher than nornmal tissue ,chronic gastitis,intestinal metaplasia and atypical hyperlasia（P<0.05）；The positive rate of SDF-1 in gastric cancer was 48.21%,that was lower than nornmal tissue ,chronic gastitis and intestinal metaplasia（P<0.05）.There was no significant difference between the positive rate of SDF-1 in gastric cancer and atypical hyperlasia（P＞0.05）. Moreover,the <WP=9>expressions of VEGF and CXCR4 in gastric cancer were correlated significantly with the depth of local infiltration of gastric ,lymph node matastasis and the clinical stage（P<0.05）,and their expressions were not associated with patient’s age, sex, tumor size and the degree of tumor differentiation（P＞0.05）. The expression of SDF-1 was not associated with clinincal pathological features of gastric cancer（P＞0.05）. The expression of VEGF was significantly related with the expression of CXCR4（γ＝0.452，P＜0.01）， but they were not related with the expression of SDF-1（P＞0.05）.Conclusion: The expression of VEGF and CXCR4 in gastric cancer was significantly higher.Besides the function of stimulating angiogenesis by paracrine machanism，VEGF possibly use autocrine signaling pathway and expression of CXCR4 to promote the invasion and matastasis of gastric cancer. The expression of SDF-1 in gastric cancer was significantly lower,and it would be a mechanism by that gastric cancer escapes human immunity,therefore,it is possible that SDF-1 has a role in the occurrence of gastric cancer.更多还原