【作者】 杨万莲；

【导师】 佘锐萍；

【作者基本信息】 中国农业大学 ， 兽医病理学， 2004， 硕士

【摘要】 本试验选用了32只体重5公斤左右的成年临床健康北京犬，随机分为模型组、茵陈蒿汤组、郁金茵陈组、茵栀黄组，每组8只，运用异硫氰酸-1-萘脂(1-Naphthyl Isothiocyanate，ANIT)经口灌服制作试验性胆汁淤积性黄疸动物模型，观察三种中药方剂对试验性胆汁淤积性黄疸的预防和治疗作用，试验期15天。结果显示： 1．北京犬的正常值的测定 测定了32只试验犬在临床健康状态下的各项血液生理学和血清学指标，提供了北京犬正常值的参考范围。 2．试验性胆汁淤积性黄疸模型的建立 ANIT经口灌服后，引起试验犬血清胆淤常规指标TB、DB、AST、ALT和ALP均明显升高，符合急性肝胆汁淤积的生物化学改变，且各项指标在灌服ANIT后第十二天已全部自然恢复正常。 3．三种中药方剂对血液白细胞的影响 灌服ANIT后，模型组白细胞迅速上升且上升期较长，第四天才达到峰值，白细胞最高值高于各中药组的白细胞最高值且差异显著(P＜0.01)，第十二天恢复至灌服ANIT前的水平；郁金茵陈组白细胞缓慢上升且最高值与灌服ANIT前差异不显著(P＞0.05)；茵陈蒿汤组和茵栀黄组白细胞总数第二天达到峰值后即开始下降，且最高值与灌服ANIT前差异显著(P＜0.01)，第七天均已恢复至灌服ANIT前的白细胞水平。说明郁金茵陈方具有良好的抗炎作用，另两组中药也具有一定的抗炎作用。 4．三种中药方剂对血清胆红素含量的影响 灌服ANIT后，各组胆红素含量迅速上升，均在第二天达到峰值，以郁金茵陈组胆红素含量最低，且恢复最快。模型组胆红素含量峰值高于三个中药组，但只与郁金茵陈组存在显著差异(P＜0.01)。与另两个中药组间差异不显著(P＞0.05)。说明郁金茵陈方的利胆退黄作用优于另两组中药。 5．三种中药方剂对肝脏功能的影响 灌服ANIT后，模型组肝脏酶活性上升最快，下降最慢，且肝脏病理变化最显著；三个中药组肝脏酶活性均不同程度的上升但均低于对照组，茵陈蒿汤组和茵栀黄组犬的肝脏酶活性高于郁金茵陈组，郁金茵陈组犬整个试验期各项酶活性均与ANIT前无差异(P＞0.05)。说明郁金茵陈方改善肝脏功能的作用优于其它两个中药组。更多还原

【Abstract】 Thirty-two adult healthy Pekingeses, with the weight of approximately 5 kg, were randomly divided into 4 groups, 8 dogs for each group. Group 1 was used as the control, and the others (group 2, 3 and 4) were used as the experimental groups and respectively given Yinchenhao decoction, Yujin Yinchengao decoction and Yinzhihuang injection. The models of intrahepatic cholestasis jaundice for all dogs were induced by oral administration of ANIT, and then treated with different preparations of Chinese herbs mentioned above for 15 days. The effects of prevention and treatment to intrahepatic cholestasis jaundice were observed for these Chinese herbs in this experiment. The results were given follows:1. To determine physiological and biochemical data in Pekingeses: Physiological and biochemical data are determined for all clinical healthy Pekingeses in the experiment, which could be used as normal biological value to evaluate clinical treatment in Pekingeses.2. Models of experimental intrahepatic cholestasis jaundice: After oral administration of ANIT, the values reflecting Pekingeses cholestasis, including serum TB, DB, AST, ALT and ALP, increase remarkably and agreet with the changes of biochemical indexs of acute intrahepatic cholestasis.All values mentioned above resume within 12 days.3. Influence of Chinese herbs on WBC: The WBC of group 1 rised rapidly and reached maximal value on the 4th day after taking ANIT, it is higer than the other groups, and the difference is significant (P<0.01). The WBC of group 3 rised slowly.cornpared with the WBC before taking ANIT, the difference is unsignificant (P > 0.05).The WBC of group 2 and group 4 reached top value on the 2th day and then decreased, compared with the WBC before taking ANIT, the difference is significant (P<0.01).This suggests that Yujin Yinchengao have remarkable anti-inflammatory, and Yinchenha soup and Yinzhihuang also have somewhat but lesser.4. Influence of Chinese herbs on serum bilirubin: Serum bilirubin of all groups rised rapidly and reached top value after administration of ANIT. Bilirubin of group 3 was relatively lowest,and resumed soonest. Although bilirubin of group 1 was the highest among 4 groups.the significant difference lied only between group 1 and group 3 (p<0.01). It indicates that Yujin Yinchengao is more beneficial on reducing serum bilirubin than the other Chinese herbs.5. Influence of Chinese herbs on liver function: Enzymatic activities in the liver of group 1 rised fastest and declined slowest, and the liver pathological tissue were the most severe. Enzymatic activities in the livers of other 3 groups rised to various degree, but lesser than group 1. Enzymatic activities in the liver of group 2 and group 4 were higher than group 3. Compared with before taking ANIT, group 3 didn’t appear difference during experimental period. These show that yujinyincheng is more better for liver function than yinchengao and Yinzhihuang.更多还原