【作者】 韩妍妍；

【导师】 王维娜； 王安利；

【作者基本信息】 河北大学 ， 水生生物学， 2003， 硕士

【摘要】 本文研究了中草药对史氏鲟(Acipenser schrencki)幼鱼的诱食活性，将诱食试验中筛选出来的4种中草药提取液添加到配合饲料中对史氏鲟进行30d的生长试验，并测定了史氏鲟血细胞中的超氧阴离子值、肌肉中的抗氧化酶活性和游离氨基酸组成。此项研究探讨了中草药诱食剂作为商业饲料的可行性，为进一步开发研究提供了资料。试验结果如下： 1．中草药复合试剂1、5、7、8等4种提取液对史氏鲟有显著引诱作用(P＜0.05)，作用效果依次是：复合试剂8＞复合试剂5＞复合试剂7＞复合试剂1。 2．将上述4种中草药提取液分别添加到基础饲料中饲喂史氏鲟，发现史氏鲟幼鱼能接受此配合饲料，且与对照组相比各试验组的生长情况都有不同程度的改善，其中试验4组尤为显著。 3．投喂中草药饲料的史氏鲟血细胞中的超氧阴离子值均比对照组有所下降，肌肉中抗氧化酶活性也有所升高。这说明中草药的确有清除自由基和抑制自由基的生成及提高抗自由基酶类活性的奇特作用。 4．投喂中草药提取液的史氏鲟肌肉中的风味氨基酸以及总游离氨基酸含量均高于对照组，这将更好的满足人们对水产品健康美味的需求。更多还原

【Abstract】 Ten kinds of complex reagents were employed to study the feeding attraction activities of amur sturgeon (Acipenser schrencki) in this experiment. The results for the attractions of complex reagents to amur sturgeon were different, only four kinds of compounds’ attracting activities were prominent (P<0.05). The four kinds of Chinese . herb medicine attractants were added into the basal feed respectively to feed amur sturgeon in the next growth test. At last, we determined the reactive oxygen intermediate values in blood and the antioxidant enzyme activities and free amino acids in muscle of amur sturgeon. The feasibility of Chinese herb medicine attractants as commercial feed is also discussed in the paper, and it provids some instructive materials for further studies on Chinese herb medicine attractants. The main conclusions of the experiment are as follows:1. The feeding attraction activities of complex reagent 1, 5, 7 and 8 were all prominent. The results were analyzed by one-way ANOVA, and the comparison of their feeding attraction activities are as follows: complex reagent 8>complex reagent 5>complex reagent 7>complex reagent 1.2. The above four kinds of Chinese herb medicine attractants were added into the basal feed respectively to feed amur sturgeon in the growth test. The results are as follows: amur sturgeon larvae could accept the feed, and the results of the test groups were better than that of the controlled group which was reared only by the basal feed.3. Reactive oxygen intermediate values in blood cell of amur sturgeon reared in the test groups were all lower than those of the controlled group. At the same time, antioxidant enzyme activities in muscle of amur sturgeon in the test groups were all higher than those of the controlled group. The results indicate that Chinese herb medicines have some magical effect on killing reactive oxygen intermediate values and enhancing antioxidant enzyme activities.4. The flavour amino acids and whole free amino acids in muscle of amur sturgeon reared by the Chinese herb medicine feed were higher than those of the controlled group. This is more helpful to meet people’s requirement to aquatic animals.更多还原