【作者】 樊明欣；

【导师】 雷治海；

【作者基本信息】 南京农业大学 ， 基础兽医学， 2003， 硕士

【摘要】 1 leptin长形受体与NPY在猪前脑内共存 用原位杂交与免疫组织化学相结合的方法研究了5头三元杂交仔猪前脑内leptin长型受体(OB-Rb)mRNA与神经肽Y(NPY)免疫反应阳性物质的共存关系。实验结果表明，leptin长型受体(OB-Rb)mRNA和NPY免疫反应阳性神经元分布于下丘脑漏斗核和前室周核、海马、杏仁核及大脑皮质。上述结构内一些表达leptin长型受体mRNA的神经元也含有NPY免疫反应阳性物质，即leptin长型受体与NPY共存于同一神经元内。试验结果提示leptin可能通过下丘脑漏斗核和室周核以及边缘系统的海马和杏仁核，甚至大脑皮质中表达leptin长型受体mRNA的NPY免疫反应阳性神经元调节动物的摄食、能量代谢、生长和繁殖等活动。 2 leptin长形受体与SS在猪前脑内共存 用原位杂交与免疫组织化学相结合的方法研究了三元杂交和梅山仔猪前脑内leptin长型受体mRNA与生长抑素(SS)免疫反应阳性物质的共存关系。结果表明，leptin长型受体mRNA和生长抑素免疫反应阳性神经元分布于下丘脑室周核、背内侧核、腹内侧核、室旁核、海马、杏仁核及大脑皮质，且在三元杂交与梅山仔猪之间分布无明显差异。上述结构内一些表达leptin长型受体mRNA的神经元也含有生长抑素免疫反应阳性物质，提示leptin可直接作用于下丘脑的生长抑素免疫反应阳性神经元调节动物的能量代谢、生长和繁殖等活动。更多还原

【Abstract】 1 Colocalization of NPY and OB-Rb mRNA in the forebrain of piglets studied by the combination of in situ hybridization and immunohistochemistryCombination of in situ hybridization and immunohistochemistry methods was used in this study to investigate coexistence of the long form leptin receptor(OB-Rb) mRNA and neuropeptide Y-like immunoreactivity(NPY-lR) in neurons of the pig forebrain. The results shown that both OB-Rb mRNA and NPY-IR containing neurons were seen in the infundibular nucleus and periventricular nucleus in the hypothalamus, the hippocampal formation, amygdala and cortex of the pig forebrain, and that some of neurons expressing OB-Rb mRNA also contained NPY-IR. It meant coexistence of OB-Rb and neuropeptide Y in these neurons of the pig forebrain. The results suggested that leptin may exert control of food intake, energy metabolism, growth and reproduction through the NPY neurons expressing OB-Rb.2 Colocalization of SS and OB-Rb mRNA in the forebrain of piglets studied by the combination of in situ hybridization and immunohistochemistryTo investigate the coexistence of the long form leptin receptor mRNA and somatostatin-like immunoreactivity(SS-IR) in neurons of the pig forebrain , combination of in situ hybridization and immunohistochemistry methods were used. The results shown that both OB-Rb mRNA and SS-1R containing neurons were seen in the periventricular, dorsomedial, ventromedial and paraventricular nuclei of the hypothalamus, the hippocampal formation, amygdala and cortex of the pig forebrain, and it seemes that there were no obvious differents on the localization between Landrace and Meishan pigs. There were also some of neurons expressing OB-Rb mRNA also containing SS-IR, that’s to say that there was coexistence of OB-Rb and SS in these neurons of the pig forebrain. So leptin may regulate food intake, energy metabolism, growth and reproduction through the SS neurons expressing OB-Rb.更多还原