【作者】 孙国荣；

【导师】 章世元；

【作者基本信息】 扬州大学 ， 动物营养与饲料科学， 2002， 硕士

【摘要】 为了研究粗制植酸酶（简称粗制酶）在不同AP蛋鸡日粮中添加的效果，用70周龄新罗曼蛋鸡进行饲养和代谢试验。测定指标包括产蛋性能、物质代谢、骨骼质量和血液生化指标。试验采用2×3因子设计，处理因素包括两个粗制酶（0、0.5％）和三个AP（0.1％、0.2％、0.4％）。试验结果如下： 1．产蛋性能 经6周的饲养试验得到：AP为0.1％时，添加本粗制酶，产蛋率增加8.61个百分点；耗料量增加0.47g/（d·只）；平均产蛋量增加6.51g/（d·只）；体增重增加2g/2wk；料蛋比下降11.65％；破蛋率下降1.22个百分点；在试验的2、4、6周，蛋壳厚度分别增加1.62×10^-1mm、2.45×10^-1mm、3.35×10^-1mm，蛋壳强度分别增加1.04kg/cm²、1.30kg/cm²、1.63kg/cm²。0.1％AP加粗制酶组的生产性能均达到或超过AP为0.4％的不加粗制酶组。 2．物质代谢 经1周代谢试验得到：AP为0.1％时，添加本粗制酶，使GE、CP、Gly、Ca、P、Fe、Mn、Zn的表观存留率分别增加3.55％、5.21％、1.72％、60.02％、329.88％、11.49％、15.72％、40.51个百分点；AP为0.2％时，添加粗制酶对这些指标也有提高，但幅度较小。添加粗制酶后，Cu的表观存留率降低，随AP的增加降低的幅度增加。AP为0.4％时，Ca、Fe、Zn的表观存留率有下降趋势。AP与粗制酶对DM、除Gly外其他AA的表观存留率影响不显著（P＞0.05）。 3．骨骼质量和血液生化指标 AP和粗制酶的对胫骨总灰重、灰分含量、钙含量及血磷、血钙、血中磷性磷酸酶活性无显著影响（P＞0.05）。低AP对骨磷含量有显著影响（P＜0.05）。当AP为0.1％时，骨P含量为10.97％，显著低于AP为0.2％、0.4％的加酶与不加酶组；添加粗制酶后，骨磷含量为17.99％，与AP为0.1％、0.2％的加酶与不加酶组差异不显著（P＞0.05）。更多还原

【Abstract】 A study was conducted to determine the effects of available phosphorus levels and crude phytase on layer.Used 70 weeks old New Loman Laying Hens,study included feeding and metabolism experiments. Criteria used were performance, nutrient retention, tibia quality and blood criteria. The treatment diets were fed in a factorial arrangement of two levels of crude phytase(0,0.5%) and three levels available P(0.1%,0.2%,0.4%).the results are as follows: PerformanceResults of 6 weeks feeding experiment are as follows: 0.1% Ap diet with 0.5% crude phytase, resulted in increased egg production by 8.61 percentage points, increased feed consumption by 2g/2wk, improved average egg yield by 6.51g/(d · bird), descended feed egg ratio by 11.65%, reduced percentage of defective eggs by 1.22 percentage points, improved eggshell thickness by 1.62× 10-1mm 2.45× 10-1mm, 3.35× 10-1 mm and strength by 1.04 kg/cm2, 1.30 kg/cm , 1.63 kg/cm during 2,4,6 weeks of feeding experiment. Performance of hens fed 0.1 %Ap diet with 0.5% crude phytase attained or exceeded that of hens fed 0.4% AP diet without crude phytase. Nutrients RetentionResults of 1 week metabolism experiment are as follows: 0.1% Ap diet with 0.5% crude phytase made apparent retention of GE, CP, Gly, Ca, P, Fe, Mn, Zn significantly(P<0.05) improved by 3.55%, 5.21%, 1.72%, 60.02%, 329.88%, 11.49%, 15.72%, 40.51 percentage points respectively.0.2% Ap diet with 0.5% made these criteria improved by smaller range. When AP level depressed, crude phytase made apparent retention of Cu reduced significantly. 0.4% Ap diet with 0.5% crude phytase had a trend.to descend the apparent retention of Ca,Fe,Mn. There was no significant effects of AP and crude phytase in apparent retention of DM and all AA except for Gly (P>0.05.) . Tibia Quality and Blood CriteriaAP and crude phytase had no significant influence on tibia ash, ash percentage, Ca content of tibia ash, plasma Ca and P, AKP activity. 0.1% Ap diet with 0.5% crude phytase creased P content of tibia ash significantly(P<0.05). P content of tibia ash of Hens fed the 0.1 %Ap diet without 0.5% crude phytase was 10.97%,significant lower than that of hens fed 0.2%,0.4% AP diet with or without crude phytase. When 0.1 %Ap diet supplemented with 0.5% crude phytase, P content of tibia ash was 17.99%,have no difference with that of diet with 0.2%,0.4% AP diet with or without crude phytase (P>0.05 ) .更多还原