【作者】 陈瑜；

【导师】 林建银； 张声；

【作者基本信息】 福建医科大学 ， 生化与分子生物学， 2003， 硕士

【摘要】 目的：探讨ANG在胃癌血管生成中的作用。 方法：运用RT-PCR技术，检测68例胃癌组织及相应癌旁胃黏膜ANG mRNA的表达水平，免疫组化技术检测相同胃癌组织CD34、Ki-67、VEGF、ANG的表达情况，根据CD34的表达情况计算出胃癌组织的微血管密度(MVD)，根据Ki-67的表达情况计算出肿瘤细胞增殖指数。 结果： 1．68例胃癌患者的原发性胃癌及癌旁组织均见有ANG mRNA的表达；癌组织总体ANG mRNA表达水平(0.482±0.094)显著高于相应癌旁胃黏膜(0.276±0.019，P=0.03)。 2．胃癌癌组织与相应癌旁胃黏膜的ANG mRNA表达水平的比值(T/N)与肿瘤组织MVD显著相关，并随着ANG mRNA表达水平的增强，MVD也随之升高，两者呈正相关(r=0.380，P=0.001)。胃癌组织ANG蛋白水平与ANGmRNA的表达水平同时升高，ANG蛋白水平与ANG mRNA的表达水平间呈正相关。随着癌组织ANG蛋白表达水平的增强，MVD也随之升高，两者呈正相关。 3．癌组织ANG蛋白表达水平与VEGF的表达显著相关，并且随着VEGF表达水平的增强，ANG蛋白水平也随之升高，两者呈正相关(P=0.009)。 4．VEGF与ANG蛋白均阳性表达组的MVD明显高于VEGF与ANG蛋白均阴性表达组的MVD，差别有显著性意义(P=0.0267)。 5．癌组织ANG蛋白表达水平与肿瘤组织中肿瘤细胞增殖指数显著相关，并且随着ANG蛋白表达水平的增强，肿瘤细胞增殖指数也随之升高，两者呈正相关(P=0.009)。 6．胃癌组织ANG mRNA、ANG蛋白表达水平、VEGF表达水平与患者年龄、肿福建医科大学硕士研究生毕业论文 瘤发生部位、组织学分型、淋巴结转移、临床分期等未见明显关系。 结论: 1.ANG mRNA在胃癌组织的表达高于相应癌旁胃薪膜。 2.ANG表达的水平升高与胃癌柳D呈正相关，ANG在胃癌组织中发挥血管 生成因子的作用，促进肿瘤性血管生成。 3.ANG与vEGF在促进胃癌血管生成中可能存在协同作用。 4.ANG表达的水平升高与胃癌细胞增殖指数呈正相关，ANG具有促进肿瘤 细胞增殖的作用。 总之，ANG参与胃癌血管生成，促进肿瘤细胞生长，进一步加深了对胃癌血管生成机制的认识，为寻找新的抗肿瘤血管生成治疗途径提供理论依据。更多还原

【Abstract】 Objective: To explore the role of ANG in angiogenesis of gastric cancer.Methods: ANG mRNA expressions of 68 gastric cancer tissues and paired adjacent gastric mucosas were detected by reverse transcript polymerase chains reaction (RT-PCR). The expressions of CD34 , Ki-67, VEGF and ANG of the same gastric cancer tissues were observed by immunohistochemistry. Microvessel density (MVD) were figured out according to their expressions of CD34. The proliferation indexes of cancer cells of gastric cancer tissues were figured out according to their expressions of Ki-67. Results:1.The expression of ANG mRNA was found both in gastric cancertissues and their paired adjacent gastric mucosas. In the 68 cases, theANG expression level of gastric cancer tissues (0.482±0.094) issignificantly higher than that of the paired adjacent gastric mucosas(0.276 ± 0.019 P=0.03) .2. Ratio of the ANG mRNA expression of the gastric cancer tissue and the paired adjacent gastric mucosa (T/N) is significantly related to the MVD. With the increase of ANG mRNA expression, MVD elevates. A significantly positive correlation between them is found (r=0.380, P=0.001) The ANG expression in gastric cancer tissues increases with the expression of ANG mRNA, and a positive correlation is found between them. With the increase of ANG expression of the gastric cancer tissues, MVD elevates,and a positive correlation is found between them.3. The ANG expression in the gastric cancer tissues is significantly related to the expression of vascular endothelial growth factor (VEGF). With the increase of ANG expression of the gastric cancer tissues , VEGF expressoin elevates. There is a positive correlation between them(P=0.009) .4. The mean MVD of the group whose VEGF and ANG are both positive is significantly higher than that of the group whose VEGF and ANG are both negative, the difference is significant (P=0.0267) .5. The ANG expression in the gastric cancer tissues is significantly related to the proliferation index of cancer cells in gastric cancer tissue. With the increase of ANG expression in gastric cancer tissue, the proliferation index of cancer cells of gastric cancer tissue elevates. There is a positive correlation between them (P=0.009) .6. The expressions of ANG mRNA, ANG and VEGF in gastric cancer have no relation to age, location of the tumor, histological type, lymph node metastasis and clinical staging of the patients.Conclusions:1. The ANG expression in gastric cancer tissues is higher than that of the paired adjacent gastric mucosa.2. In gastric cancer, there is a positive correlation between ANG expression level and MVD. ANG acts as an angiogenic factor which promotes the growth of the carcinomatous blood vessel.3. ANG may work in coordination with VEGF in promoting the angiogenesis of gastric cancer.4. There is a positive correlation between ANG expression and tumor cell proliferation index in gastric cancers. ANG may have the function to promote the growth of the cancer cells.In a word, this study helps to further understanding the mechanism on angiogenesis of gastric cancer and provides theoretical reference to explore a new way of antiangiogenesis in gastric cancer.更多还原