【作者】 戴琦；

【导师】 李敏； 吴秀山； 袁婺洲； 梁宋平；

【作者基本信息】 湖南师范大学 ， 生物化学及分子生物学， 2001， 硕士

【摘要】 最近的研究表明，果蝇的早期心脏发育模式与脊椎动物及人的心脏早期发育模式具有惊人的相似性。阐明果蝇心脏发育的基因控制机理有助于揭示人体心脏发育机理和先心病发生机制。为了克隆和鉴定控制心脏发育的新基因，本研究利用化学诱变剂甲磺酸乙酯大规模地诱变果蝇，并且建立了1200个第二和第三染色体的平衡致死系，利用心脏组织特异抗体Mab.No.3对其中330个品系进行免疫化学方法筛选，观察到有60个致死系表现出心脏突变表型，20个品系的心脏突变表型有待进一步证实。分别将这些有心脏突变的品系与果蝇第二和第三染色体缺失系杂交，测定了11个品系的遗传学位点，其中4个品系在遗传学位点上有别于已经报道的心脏发育控制基因。根据缺失区段，在果蝇数据库中检索了7个品系相关的可能候选基因，它们的功能主要有转录因子、翻译因子、激酶、和信号分子等。这些影响心脏突变表型的候选基因还需要进一步鉴定。同时，利用P因子诱变，建立了一个心脏基因的P因子等位基因新平衡致死系。更多还原

【Abstract】 Recent studies indicate that there is a remarkable similarity in early heart development pattern among Drosophila, vertebrate and human. Studies of genes related to heart development in Drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases. To clone and identify new genes that control the heart development, by a way of chemical mutagen ,EMS, we have established 1,200 balanced-lethal lines on chromosome 2 and 3.With the screening the 330 stocks with immunochemical method using heart-specific antibody, Mab.No.3, we detected 60 lethal lines showing heart mutant phynotype. The mutant lines were crossed with deficiency lines on chromosome 2 and 3 respectively, and the genetic sites of 11 stocks have been determined. Of them four showed a different phenotype from the known genes that control the heart development. Based on the deletion region, the possible candidate genes of 20 stocks were searched in flybase. They mainly function as transcription factors, translation factor, kinase, signal molecules, and so on. These candidates affecting heart mutant phenotype are to be identified. At the same time, a mutant stock has been established with P element mutation.更多还原