无特定抗原刺激大鼠乳腺免疫细胞的研究

【作者】 朴正浩；

【导师】 俞曙林；

【作者基本信息】 延边大学 ， 基础兽医学， 2003， 硕士

【摘要】 本研究采用组织化学技术，研究在无特定抗原刺激下大鼠乳腺免疫细胞的变化，探讨了泌乳期和静止期乳腺免疫的特点。 本研究结果表明，泌乳期和静止期大鼠乳腺组织中只存在黏膜型肥大细胞，肥大细胞大小没有明显的差异(P＞0.05)；肥大细胞数泌乳期为2.8±0.9个/视野，静止期为9.9±1.8个/视野，两组差异极显著(P＜0.01)。泌乳期乳腺组织中淋巴细胞数为11.7±2.1个/视野，静止期为19.6±3.0个/视野，两组比较差异显著(P＜0.05)；泌乳期外周血涂片中淋巴细胞数为1.6±0.4个/视野，静止期为1.5±0.7个/视野，两组比较差异不显著(P＞0.05)。泌乳期乳腺组织中ANAE~+T细胞数为6.7±0.2个/视野，静止期为11.4±0.6个/视野，两组比较差异极显著(P＜0.01)；泌乳期外周血涂片中ANAE~+T细胞数为1.1±0.3个/视野，静止期为0.8±0.2个/视野，两组比较差异不显著(P＞0.05)。泌乳期CD4~+T细胞数为3.1±0.8个/视野，静止期为4.4±0.4个/视野，两组没有显著性差异(P＞0.05)；泌乳期CD8~+T细胞数为1.3±0.3个/视野，静止期为2.7±0.5个/视野，两组有显著性差异(P＜0.05)；泌乳期CD4~+/CD8~+比值为2.4，静止期为1.6。泌乳期乳腺组织中浆细胞数为1.3±0.3个/视野，静止期为1.3±0.4个/视野，两组没有显著差异(P＞0.05)。乳腺组织中IgA~+浆细胞免疫组化实验呈阴性反应。 由此可知，在无特定抗原刺激条件下，大鼠乳腺肥大细胞是黏膜型肥大细胞；静止期乳腺的细胞免疫比泌乳期高；乳腺免疫具有相对独立性；泌乳期乳腺免疫状态高于静止期；乳腺体液免疫没有随生理时期而发生变化。更多还原

【Abstract】 This study explore the immune characteristic of the rat mammary gland and immune cell in lactating period and non-lactating period by histochemical techniques.The results showed: Only mucosal mast cells(MMC) distributed in rat mammary gland without connctive mast cells.These MC sizes were similar(P>0.05).The MC numbers per field(by 40x object lens, same as the following) were 2.8 0.9 and 9.9 1.8 respectively during lactating and non-lactating periods. The number remarkably increased in dry stage(P<0.01). The lymphocytes number during lactating and non-lactating periods is respectively 11.7 2.1 and 19.6 3.0, the former have significant difference from the latter(P<0.05), without no difference to lymphocytes, respectively 1.6 0.4 and 1.5 0.7 in the two stages in peripheral blood(P>0.05). Mammary gland ANAE+ T cell numbers varied from 6.1 0.2 in lactating stage to 11.4 0.6 in dry stage(P<0.01), while ANAE+ T cell numbers in peripharal blood varied from 1.1 0.3 to 0.8 0.2 (P>0.05). CD4+ T cell numbers varied from 3.1 0.8 to 4.4 0.4 and CD8+ T cell numbers varied from 1.3 0.3 to 2.7 0.5.The former hadn’t significant difference(P>0.05) but the latter significantly increased(P<0.05). CD4+/ CD8+ value reduced from 2.4 in lactating stage to 1.6 in non-lactating stage. The mammary gland plasmacyte numbers were 1.3 0.3 in lactating stage and 1.3 0.4 in dry stage. They were nearly equal to each other(P>0.05).No IgA+ lasmacytes existed in the rat mammary gland by immunohistochemical method.In conclusion, the rat mast cells belonging to mucosal mast cells without specific antigen .The level of cell immune is enhanced in non-lactating not in lactating period, although the whole immune level is great high in lactating period.. The immune of mammary gland may be a independence system. The immune properties and immune level obviously varied in physiological cycle更多还原