【作者】 周美娟；

【导师】 丁振华；

【作者基本信息】 中国人民解放军第一军医大学 ， 防原医学， 2003， 硕士

【摘要】 目的 探讨湿热环境（HHE）对辐射损伤动物的生存率、中位生存时间等的影响规律，并对其损伤机制做初步探讨。方法 1．将实验动物分别分为室温（RT）组和不同条件的HHE组，各组先分别给予5.0、7.0、9.0Gy的Co-60γ射线一次全身均匀照射，RH组动物置于RH环境下饲养，HHE组则先在HHE下作用相应的时间后也置于RH环境下饲养，观察小鼠30d的存活情况获取生存指标的变化规律；2．给予RH组和HHE组小鼠半数致死剂量(LD_50/30)6.5GyCo-60γ射线一次全身均匀照射后，在照后第1、3、5、9、15d取小鼠外周血、骨髓有核细胞、脾脏、胸腺，观察外周血象、骨髓有核细胞数（BMC）及DNA含量、脾体比（SVBWR）、胸体比（TVBWR）等变化反应以探讨HHE对辐射损伤动物的影响机制。结果1．HHE60min以上的作用时间增加受亚致死剂量（7.0Gy）照射小鼠的死亡率、缩短平均存活时间、使中位生存时间提前（P＜0.05）；而受致死剂量（9.0Gy）照射的小鼠，HHE作用组和RT组之间各指标没有无显著差异（P＞0.05）；2．HHE作用加重辐射损伤小鼠脾脏和胸腺的萎缩，使脾体比（P＜0.05）、胸体比（P＜0.05）下降；3．HHE作用加重辐射损伤小鼠的骨髓破坏，使BMC（P＜0.05）、有核细胞DNA含量（P＜0.05）下降；4．HHE对辐射损伤小鼠外周血象的影响：外周血白细胞、淋巴细胞、红细胞、血红蛋白变化无显著差异（P＞0.05），血小板数量在HHE组降低，差异显著（P＜0.05）。结论 HHE加重辐射损伤动物的损伤，增加死亡率，其作用机制之一是通过加重辐射损伤小鼠造血损伤以及脾脏、胸腺的损伤而引起的，其更深一步的机制有待进一步探讨。更多还原

【Abstract】 Object To study the effect and preliminary mechanism of hot and humid environment on v radiation irradiated mice. Methods 1. After being irradiated by V radiation at the dosage of 5.0,7.0 or 9.0 Gy, the mice were treated with hot and humid environment (HHE) or room temperature (RT) respectively. The survival index was then obtained by calculating the death rate within 30 days. 2 .After being irradiated by Co-60 Y radiation at the dosage of median lethal dose in 30 days(LDso/3o, 6.5 Gy) the peripheral blood ,bone marrow cell, spleen and thymus of both RT and HHE groups were examined at the day post-irradiation 1,3,5 ,9 and 15 days respectively. The blood rountine, bone marrow cellularity (BMC), the DNA content of BMC, spleen vs body weight ratio(SVBWR), thymus vs body weight ratio(TVBWR) were analyzed in order to find out the mechanism of changes in HHE. Result 1 Compared to the mice treated with RT, there is a distinguish difference between the death rate, average survival time and the met-survival time when the mice were treated with HHE more than 60min after being irradiated by Y radiation at the dosage of 7.0 Gy (P<0.05), but was not the dosage of 9.0 Gy (P>0.05), 2 The atrophy of spleen and thymus was more serious in the mice treated with RT than the mice treated with HHE, and SVBWR, TVBWR have obvious reduced(P<0.05), 3 The BMC and DNA content of BMC were obviously reduced (PO.05) in the mice treated with HHE. 4 The changes of blood rountine in the HHE group compared to the RT group: peripheral blood white blood cell, lymphocytic cell, red cell and hemoglobin have no obvious difference (P>0.05), but there is a significantly decreased in blood platelet (P<0.05). Conclusions HHE aggravated the radiation damage and increase the death rate. One of the mechanisms may be that HHE make the radiation induced bone marrow damage, hematopoietic destruction, spleen and thymus injury aggravated.更多还原