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四种爬行动物消化道内分泌细胞的免疫组织化学研究
Immunohistochemical Studies on Endocrine Cells in the Digestive Tract of Four Reptiles
【作者】 黄徐根;
【导师】 吴孝兵;
【作者基本信息】 安徽师范大学 , 动物学, 2003, 硕士
【摘要】 本文应用七种胃肠激素抗血清分别对石龙子、多疣壁虎、乌龟和红耳龟消化道内分泌细胞的形态、分布进行了免疫组织化学研究。在四种爬行动物消化道所含内分泌细胞中,5HT-IR细胞是分布最广的一种,其在四种动物消化道各段均有分布,但分布型各不相同; SS-IR细胞在多疣壁虎和乌龟消化道中主要分布于其前段,而在石龙子和红耳龟中则分布于整个消化道中;Gas-IR细胞在几种动物消化道中主要集中分布于胃幽门部或(和)十二指肠部位;Glu-IR细胞与SP-IR细胞在四种动物消化道中分布型差异较大;PP-IR细胞仅在红耳龟消化道中有少量分布; Ins-IR细胞在四种动物消化道中均未见。几种爬行动物消化道内分泌细胞的分布既表现出了一定的共性,体现了爬行类生活习性及消化生理的某些共同点,又表现出不同物种对各自生活环境、食性很强适应性的种间差异。
【Abstract】 Identification, distribution and morphology of edocrine cells in the digestive tract of Enmeces chinensis 、Gekko japonicus 、Trachemys scripta and Chinemys reevsii were studied by immunohistochemical method with seven gut hormone antisera. 5HT-IR cells is the only one that extensively distributed in whole digestive tract of four reptiles which distributive mode were different from each other. SS-IR cells were presented in the former part of digestive tract of Chinemys reevsii and Gekko japonicus, however they distributed throughout the digestive tract of Enmeces chinensis and Trachemys scripta. Gas-IR cells were mainly occurred in the stomachus pyloricus or (and)duodenum of four reptiles. The distributive mode of Glu-IR cells and SP-IR cells in the digestive tract were different among four reptiles.PP-IR cells were only rarely detected in the digestive tract of Trachemys scripta . Ins-IR cells were not detected in any of four reptiles. Some common features in identification, distribution and morphor of endocrine cells were found in 4 reptiles, which exist in digestive physiology and life habit. But the differences among 4 reptiles were also significant, which exist in the adaptability to feeding habit and life environment respectively of each reptiles.
【Key words】 Reptiles; Digestive tract; Edocrine cells; Immunohistochemistry;
- 【网络出版投稿人】 安徽师范大学 【网络出版年期】2004年 01期
- 【分类号】Q952
- 【被引频次】3
- 【下载频次】358