【作者】 池爱平；

【导师】 熊正英；

【作者基本信息】 陕西师范大学 ， 运动人体科学， 2003， 硕士

【摘要】 大强度运动时，机体会产生大量的自由基。当机体本身的自由基防御系统不足以及时清除这些自由基时，它们便开始攻击机体生物大分子，破坏机体细胞和组织以及生物膜系统，引起生物膜发生脂质过氧化反应，导致蛋白质变性、线粒体功能障碍等等，使细胞正常的功能受到破坏，进而引发运动疲劳的产生，使运动能力下降。姜黄素是从姜科姜黄属植物姜黄根茎中提取的一种酚性色素，其分子结构特点是同一分子结构中具有酚及β-二酮结构。它不仅有很强的抗氧化性，而且在抗氧化过程中生成的产物是稳定性很好的醌物质。由于其色泽稳定、价格便宜、且毒性极低，目前被广泛应用于食品添加剂和染料中。它以保护正常细胞免受各种不良因素的损伤为基础，发挥其抗炎、抗肿瘤、抗氧化、抗病毒等药理作用。本实验给大鼠灌服姜黄素溶液，目的在于揭示姜黄素对大强度耐力训练大鼠的生长发育、能量与物质代谢和各组织的自由基防御体系的影响，以及对红细胞膜抗氧化能力、转运K⁺、Na⁺的能力的影响，为姜黄素在运动医学领域中的应用和运动保健食品的开发提供实验根据。 本实验以SD雄性大鼠为实验对象，使用递增强度跑台训练，建立大强度耐力运动模型。训练一段时间后，测定心系数、肝指数、脾指数和胸腺指数以反映姜黄素对运动大鼠的生长发育的影响：测定了主要血清酶及反映大鼠物质与能量代谢的一些生化指标；测定了各组织的SOD活性、Cu，Zn-SOD活性、MDA含量、CAT活性、GSH-Px活性等反映自由基防御系统的指标；测定了运动大鼠的红细胞膜SOD活性、CAT活性、GSH-Px活性、Na⁺，K⁺-ATP酶活性、Ca²⁺-ATP酶的活性和MDA的含量，以反映红细胞膜的抗氧化能力、转运K⁺、Na⁺的能力的指标。 实验结果表明：（1）姜黄素能改善大鼠由于大强度耐力训练造成的血红蛋白（Hb）含量下降的情况，使大鼠血红蛋白（Hb）含量明显上升；（2）姜黄素组运动大鼠血清ALT、AST、CK、LDH活性明显低于运动对照组：姜黄素组运动大鼠血清LDL、BUN含量都较运动组有显著的降低性；姜黄素组运动大鼠血清Cr、HDL的含量较运动对照组又有显著升高；（3）服用姜黄素运动大鼠肌糖原和肝糖原含量都明显高于运动组；（4）姜黄素组运动大鼠红细胞膜SOD活性、CAT活性、GSH-Px活性、Ca⁺-ATP酶活性和Na⁺，K⁺-ATP酶活性较运动组都有显著性升高；（5）姜黄素对运动大鼠血浆、心肌组织、肝组织、脑组织、肾脏组织、骨骼肌都表现出明显的抗脂质过氧化的作用和清除运动产生的过剩自由基的能力。 结论：姜黄素有提高有氧运动能力的作用；姜黄素能减轻大强度耐力运动对大鼠肝脏、心肌、骨骼肌、肾脏等组织细胞的损伤：姜黄素能降低运动大鼠体内蛋白质分解程度，保持了肌力，使运动大鼠机体功能正常发挥；姜黄素在一定程度上增加了运动大鼠的肌酸和磷酸肌酸含量，提高运动大鼠肌肉的工作能力；姜黄素与运动结合更有效的调整血清甘油三酯、胆固醇、高密度脂蛋白和低密度脂蛋白的代谢，对心血管系统有一定的保护作用；姜黄素能够保证大强度运动时运动大鼠神经、肌肉等组织的糖供应，从而提高运动大鼠的运动能力；姜黄素能从整体提高运动大鼠的抗氧化水平，从而延缓大鼠运动性疲劳的产生；姜黄素在一定程度上提高了运动大鼠红细胞膜的抗氧化能力、转运K＼Na＋的能力，姜黄素同时也提高了运动大鼠红细胞膜的稳定性、流动性，姜黄素能保护或降低运动大鼠红细胞在大强度运动中的造成的损伤。更多还原

【Abstract】 Many studies show that exercise can cause body to produce lots of free radicals that will attack cellular membrane system and result in the damage of structure and the malfunction of cells. Curcumin is a kind of the efficient, non -poisnous and natural antioxidant. The study that is related to the antioxidic function of Curcumin has been progressing of made pleasure researching achievements in many fields such as foodstuff, medocome, cosmetology, etc. The purpose of this study is to investigate the effects of Curcumin on growth and development, free- radical- defense system, exercise performance and concentrations of RBCM, put up some reference for explortion of Curcumin in the field of the exercise medicine and the development related to Curcumin of sports food and beverage.This studie’s objects are SD rats ( ) ,The exercise models were set up by progressing intensity endurance training,The coefficient of the heart ,me indices of the liver, spleen, thymas gland were tested to investigate the effect of curcrmin on the growth and development of the athletic rat. The activity of SOD - CAI\ GSH-Px and the content of MDA were measured that reflect the status of free- radical-defense system. It was measured that some indices in the blood which reflect the metabolism of protein and performance of the aerobic exercise.The results show: 1) Curcumin can increase the indices of organs and don’t affect badly the growth and development of the rat. 2) The Hb content lightly decreases in the rals after the intensity endurance training, while Curcrmin can improve this phenomenon and make the Hb content increase remakedly, which proves that Curcumin can increase the perfomance of the aerobic exercise.3)The activity of ALT , AST, LDH, Ck and the content of LDL. BUN in the serum are lower singnifcantly in Curcumin groups than in control groups .The content of Cr and HDL in the serum is higher evidently in Curcumin groups than in control groups. These results present that Curcumin can reduce destroy of the tissues and cells caused by the intensity endurance exercise such as the liver, myocardium, and so on. 4) The concentrations of the blood - glucose and glycose in liver and myocardium are higher prominently-in Curcumin guoups than control ones .It is infered that Curcumin can raise the glycoexis in the rat body.provide thi energy to the tissues of central neuronsystem, the muscle, the red cell, etc, delay the the central and peripheral fatigue and promote performance of the exercise.5) Curcumin can improve ATPase activity in intensive endurance rats which suggest Curcumin has important significance to remain osmotic Pressure balancemand crossing membrance potential. 6) Curcumin indicates the prominent performance of antioxidation and clearing the free radicals in all the tissues of the heart, liver, kidney , brain and muscle. Then the abilities of its antioxidation are different in the different tissues in no uniform conditions, and its effect on the activity of different the antioxidic enzyme is different in the same tissue in the same conditions.The conclusion: Curcumin has the strong actions on enhancing antioxidant, clearing the free radicals, It can modify the some bad effects of endurance exercise on the body and promote the capability of exercise.更多还原