【作者】 罗立廷；

【导师】 方之平；

【作者基本信息】 华中农业大学 ， 基础兽医学， 2003， 硕士

【摘要】 用链霉亲和素—生物素化过氧化物酶复合物(Strept Avidin Biotin-peroxidase Complex，SABC)免疫细胞化学方法，使用兔抗促性腺激素释放激素(Gonadotropin-releasing hormone，GnRH)、促性腺激素释放激素受体(Gonadotropin-releasing hormone receptor，GnRH受体)、5-羟色胺(5-Hydroxytryptamine，5-HT)、5-羟色胺受体(5-Hydroxytryptamine receptor，5-HT受体)、生长激素(Growth hormone，GH)、生长抑素(Somatostatin，Som)和鼠抗黄体生成激素(luteinizing hormone，LH)、促卵泡激素(follicle-stimuling hormone，FSH)8种抗血清对性成熟前后黄颡鱼(Pelteobagrus fulvidraco)和鲶鱼(Silurus asotus)的脑、垂体、性腺和消化系统的内分泌细胞进行了研究。实验结果表明：性成熟前后，GnRH、GnRH受体、5-HT、5-HT受体和Som在两种鱼的脑、垂体、性腺和消化道中均有分布；LH和FSH在两种鱼的脑、垂体、性腺有分布；GH在鲶鱼的垂体和消化道与胰岛中有分布，GH仅在黄颡的垂体中存在；性成熟后两种鱼下丘脑、垂体和性腺的GnRH、GnRH受体、LH、FSH免疫反应细胞数目和反应强度明显高于性成熟前，其它部位变化不明显；性成熟前后两种鱼GH、Som、5-HT和5-HT受体阳性反应细胞变化不明显。在免疫细胞化学的基础上，用原位杂交的方法来检测GnRH-mRNA在两种鱼性腺成熟前后体内的分布情况。结果是GnRH-mRNA分布于脑、性腺和消化道中，其中在下丘脑的性成熟后的阳性细胞数目和反应强度明显高于性成熟前。由此我们认为GnRH、GnRH受体、LH、FSH两种鱼的性腺发育成熟起着重要的调节作用。本实验为下丘脑—垂体—性腺轴和神经—内分泌的研究提供了有力的形态学和分子水平的依据。更多还原

【Abstract】 An investigation using immunocytochemical technique of the strept avidin biotin-peroxidase complex(SABC) method was made in order to examine the identification and localization of the endocrine cells of brain, pituitary, gonad and digestive system in two kinds of siluriformes fish:Yellow catfish( Pelteobagrus fulvidraco) and Oriental sheatfish(Silurus asotus). Eight antiseras of rabbit anti-gonadotropin-releasing hormone (GnRH), anti-gonadotropin-releasing hormone receptor (GnRHR), anti-5-Hydroxytryptamine (5-HT), anti-5-Hydroxytryptamine receptor (5-HTR), anti-somatostatin (Som), anti-Growth hormone (GH) and mouse anti-luteinizing hormone(LH) and anti-follicle stimuling hormone(FSH) were used. The result showed that: in sexually immature and mature stage, positive reactions with antiseras against GnRH, GnRHR, 5-HT, 5-HTR were found in brain , pituitary, gonad and digestive tract; positive reactions with FSH, LH were found in brain , pituitary, gonad; positive reactions with GH were found in brain, pituitary, gonad pancreas and digestive tract in P. fulvidraco; and in S. asotus positive reactions with GH were found only in pituitary. The number of immunoreactive cells and positive reactions of GnRH, GnRHR, LH and FSH in the hypothalamus, pituitary and gonad in the pituitary of two fish were significantly higher in sexually mature stage than in immature stage and no distinct changes were observed in other parts in the body. The positive reactions of Som, GH, 5-HT and 5-HTR in the ovary in the hypothalamus and ovary hadn’ t been detected differences in other parts. On the basic of immunocytochemical, in site hybridization was used in order to in examine the identification and localization of the GnRH-mRNA cells in the body of two fish. The result was that: localization and expression of GnRH-mRNA was found in brain, pancreatic, gonad and digestive tract; the positive reaction was significantly higher in sexually mature stage than in immature stage. So we thought that GnRH, GnRHR, LH and FSH play an important pole in themodification of gonadal development of P. fulvidraco and S. asotus. Those results provided morphological basis and molecular basic for the research of brain-pituitary-gonadal axis and neuro-endocrine system.更多还原