【作者】 胡长敏；

【导师】 丁明星；

【作者基本信息】 华中农业大学 ， 临床兽医学， 2003， 硕士

【摘要】 目的 探讨4种不同麻醉方法下手术对犬应激性神经内分泌及免疫反应的影响，寻求术后动物免疫抑制发生发展的规律，为减轻术中应激反应及术后免疫抑制提供理论依据。方法24只杂种土犬随机分为4组：静松灵麻醉组（Ⅰ组）、盐酸氯胺酮麻醉组（Ⅱ组）、静松灵与氯胺酮复合麻醉组（Ⅲ组）、盐酸普鲁卡因硬膜外腔麻醉组（Ⅳ组）。分别在4种麻醉方法下施行外科手术，测定麻醉前0min、手术切皮后30min、术后4h、术后1d、术后3d、术后7d、术后15d血液常规、血清球蛋白含量、红细胞C₃b受体花环（RBC·C₃bR）率、T淋巴细胞转化率、血清皮质醇（cortisol）浓度及血浆β-内啡肽（β-EP）浓度。结果 各组在麻醉手术后4h及术后1d白细胞总数、嗜中性分叶状粒细胞数显著增加（P＜0.05），淋巴细胞数减少；血清球蛋白含量先降低后升高；Ⅰ组RBC·C₃bR花环率先降低再升高后降低，其它组均先降低；T淋巴细胞转化率均呈现不同程度的降低，在术后7d左右恢复正常；血浆皮质醇浓度先升高后降低，但Ⅰ组皮质醇含量在切皮后30min较其它组上升显著（P＜0.05）；血浆β-EP浓度先升高后降低，但Ⅳ组β-EP含量在切皮后30min较其它组上升显著（P＜0.05）。结论 不同麻醉方法下手术都使犬围术期免疫功能受到不同程度的抑制，但在15d左右恢复正常。神经内分泌系统和免疫系统间存在双向调节，交互影响的机制。复合麻醉可减轻麻醉手术术中、术后血浆皮质醇及β-EP浓度的升高及机体免疫功能的抑制。更多还原

【Abstract】 Objective To study the effects of operation by different anesthetic techniques on canine neuroendocrine and immune responses to stress , discuss mechanisms of immune depression at postoperation and provide therotical bases for alleviating stress reaction and immune depression during and after operation. Methods 24 crossbreed canine were alloted randomly into four groups: Xylazole general anesthesia (Group I ), Ketamine general anesthesia (Group II ), combination anesthesia of Xylazole and Ketamine (GroupIII), epidural anesthesia with Procaini hydrochloridi(Group IV) . White blood cell and its differential counts were carried out respectively before anesthesia induction , half an hour following incision , and at 4h , 1 st , 3rd , 7th and 15th after operation. At the same time , concentrations of globulin, cortisol and -EP, erythrocyte C3b receptor garland rates(RBC C3bRR), T lymphocyte blastogensis rates were measured respectively. Results White blood cell and neutrophil counts in every group increased at 4h after operation and at the 1st day after operation (P<0.05 ) , meanwhile lymphocytes decresed. Globulin content in every group decreased . RBC C3bRR in each group decreased and then increased except for Group I . T lymphocyte blastogenesis rates in each group decreased. Serous cortisol content in each group increased ,while cortisol content in Group I was higher than that in each other group (P<0.05) . Serous -EP content in each group increased and then decreased gradually, but -EP content in Group IV has a significant increased at half an hour following incision (P<0.05) .Conclusions Canine immune functions are suppressed by operation with different anesthetic techniques to different degree,but resume at 15th day after operation. There is an interacting and interadjusting mechanism among the CNS (central nerve system), the endocrine system and the immune system. Combination anesthesia of Xylazole and Ketamine can reduce stress response to anesthesia and operation, and immune depression caused by them.更多还原