茯苓增强老年大鼠皮肤中羟脯氨酸含量和SOD活性的实验研究

【作者】 于凌；

【导师】 吴景东；

【作者基本信息】 辽宁中医学院 ， 中医基础理论， 2003， 硕士

【摘要】 目的：探讨茯苓对老年大鼠红细胞中超氧化物歧化酶(SOD)活性，皮肤中SOD活性及羟脯氨酸含量的影响，判断茯苓延缓皮肤衰老的作用，并进而推测其生物学机制。原理：本项实验研究依据中医脾虚衰老的理论，中药茯苓健脾益心的功效以及现代医学中的自由基衰老学说和交联衰老学说。材料与方法：将Wistar雌性大白鼠分为青年对照组，老年空白组，阳性对照组，茯苓低剂量组，茯苓中剂量组，茯苓高剂量组，并分别予以生理盐水NS(20ml／kg)，NS(20ml／kg)，维生素E(2g/kg)，茯苓提取液2g/kg,4g／kg,8g/kg灌胃，33天后检测大鼠红细胞中及皮肤中SOD活性，皮肤中羟脯氨酸含量的变化。结果：青年对照组，阳性对照组和茯苓高剂量组大鼠皮肤的羟脯氨酸含量均高于老年空白组(p＜0.05)。茯苓低剂量组，茯苓中剂量组，茯苓高剂量组中的红细胞及皮肤中SOD活性与老年空白组比较无显著性差异(p＞0.05)。结论：茯苓可能是通过提高皮肤中羟脯氨酸的含量来延缓皮肤的老化过程，而对大鼠皮肤中及红细胞中的SOD活性没有影响。更多还原

【Abstract】 Objective: To investigate Poria’s influence on the content of hydroxvproline(HPY) in the skin of aged rats as well as the activity of Superoxide Dismutase (SOD) in both rat’s skin and red blood cells, and to infer Poria’s function and mechanism of rotarding the senility of skin. Principle: This experiment is based on the theory of spleen deficiency-skin senility and Poria’s effect in Traditional Chinese viedicine, Free Radical Theory and Cross-linking Theory in modern medicine as well. Materials and Methods: Female Wister rats were divided into six groups randomly and gavaged respectively, that is Young Control Group (NS 20ml/kg), Old Control Group (AS 20ml/kg), Positive Control Group (Vitamin E2g/kg), Low-Poria-Dosage Group (Poria 2g/kg), Medium-Poria-Dosage Group (Poria 4g/kg), High-Poria-Dosage Group (Poria 8g/kg). Peria, a kind of spleen-reinforcing herbal, is used in this study. After 33 days gavages, all the rats were killed to detect the changes of activity of SOD in both rat’ss skin and red blood cells and the content of HPY in rat’ s skin in all groups. Results: The content of HPY in the skin of Young Control Group, Positive Control Group and High-Poria-Dosage Group are higher than that of Old Control Group (p<0.05). The activity of SOD (both in red blood cell and in skin) in Poria Groups have no great changes compared with that of Old Control Group (p>0. 05). Conclusions: Poria probably have the function of increasing the content of HPY in the aged rats’ skin while it has no influence on the activity of SOD in red blood cell or derma of aged rats.更多还原