【作者】 尹昭；

【导师】 李明； 王廷华；

【作者基本信息】 昆明医学院 ， 人体解剖学与组织胚胎学， 2003， 硕士

【摘要】 [目的]：探讨八种多肽生长因子NGF、BDNF、NT-3、NT-4、GDNF、bFGF、IGF和EGF在成年猴脊髓的表达分布情况，为成年猴脊髓存在上述生长因子的表达提供可靠的免疫组织化学证据。 [方法]：云南成年健康雄性猕猴5只，平均体重6kg左右，氯氨酮（0.25ml／kg）肌肉注射麻醉后，Zamboni液经心脏主动脉插管灌注固定动物，取T₈脊髓节段入4％多聚甲醛后固定（4℃）6小时，修块后入20％sucrose-PBS液沉底。其后在-20℃恒冷切片机下制作20μm厚的连续冰冻切片，间隔取片后分别以抗NGF、BDNF、NT-3、NT-4、GDNF、bFGF、IGF和EGF抗体行免疫组化ABC染色。在Olympus光学显微镜下观察各生长因子在猴脊髓的表达分布情况。 [结果]：1．NGF、BDNF、NT-3、NT-4、GDNF、bFGF、IGF和EGF的免疫阳性反应产物在成年猴脊髓腹角神经元均有分布。2．NGF、BDNF、NT-3、NT-4、GDNF、bFGF、IGF和EGF的免疫阳性反应产物在成年猴脊髓背角神经元均有分布。3．BDNF、NT-3和GDNF的免疫阳性反应产物在猴脊髓Clarke核神经元有分布。4．NGF、BDNF、GDNF、bFGF、IGF和EGF的免疫阳性反应产物在猴脊髓背角Lissauer束均有分布，表达上述生长因子的Lissauer束因染色而呈明显的毛刷状／发束状。5．NGF、BDNF和GDNF阳性神经膨体分布于猴脊髓背角Ⅰ、Ⅱ板层。6．NGF、BDNF、NT-3、NT-4、GDNF、bFGF、IGF和EGF的免疫阳性反应产硕士研完生学位奋文多从生长困于在成年拱奋健的衣达一免度组叔化李研究物在成年猴脊髓中央管（X板层）均有分布。[结论]:成年猴脊髓存在多肤生长因子NGF、BDNF、NT一3、NT一4、GDNF、bFGF、IGF和EGF的表达，上述多肤生长因子在成年猴脊髓的分布既具有相当程度的重叠又存在一定程度的分离。提示多肤生长因子在成年猴脊髓的生理过程中发挥作用，而且这种作用具有复杂性和多面性。更多还原

【Abstract】 [Objective]: To explore the specific expression of eight kinds of polypeptide growth factors, including NGF, BDNF, NT-3, NT-4, GDNF, bFGF, IGF and EGF in the spinal cord of adult monkey. Furthermore, it’ s the first time for us to provide the immunochemistry evidences of these growth factors in the spinal cord of adult monkey for the neuroscience world.[Materials and Methods]: Five healthy adult Rhesus macaques(male) of YunNan, whose mean weight is 6kg or so, have been employed in our experiment. After being anesthesied by muscle injection of Ketamine (0.25 ml/kg), these animals were sacrificed via a way of infusing Zamboni fluid into aorta with the help of aortic cannlation. Then fixed the T8 spinal cord segments taken out from the monkeys with 4% paraformaldehyd reinst for 6h (4℃) . And put them into 20%sucrose-PBS overnight, lastly, the spinal cord were made into frozen sections in 20 μm thickness. Next, anti-NGF, BDNF, NT-3, NT-4, GDNF, bFGF, IGF and EGF serum were applied respectively with the immunohistochemical ABC method to detect the distribution of these growth factors in the spinal cord sections of adult monkey (These sections were all selected randomly.) At last, the distribution of these growth factors in the spinal cord were all observed with the help of olympus light microscope. [Results]: 1. The immunoreactivities for NGF, BDNF, NT-3, NT-4, GDNF, bFGF, IGF and EGF-like could be seen in spinal motoneurons of the adult monkey. 2. The NGF, BDNF, NT-3, NT-4, GDNF, bFGF, IGF and EGF-likeimmunoreactivities could also be seen in spinal sensory neurons of the adult monkey. 3. The positive reaction of BDNF, NT-3 and GDNF could be observed in Clarke neurons of the adult monkey . 4. The distribution of NGF, BDNF, GDNF, bFGF, IGF and EGF could be detected in the Lissauer tract of the adult monkey, as a result, the positive staining of Lissauer tract looked like hairbrush . 5. The positive varicosities of NGF, BDNF and GDNF could be seen in the spinal lamina I/II of the adult monkey. 6. The NGF, BDNF, NT-3, NT-4, GDNF, bFGF, IGF and EGF-like immunoreactivities could be found in the spinal central canal of the adult monkey.[Conclusions]: In this study, we provide the first morphological report that there exists the expression of such polypeptide growth factors as NGF, BDNF, NT-3, NT-4, GDNF, bFGF, IGF and EGF in the spinal cord of adult monkey. The spatial distributions of these growth factors in the adult monkey’s spinal cord showed both overlapping and discrepancy to some extent. This indicated that polypeptide growth factors may play multifunctional and complex roles in the spinal cord of adult monkey.更多还原